HPSE antibody (Middle Region)

Catalog No. ABIN3042457

This Rabbit Polyclonal antibody specifically detects HPSE in WB. It exhibits reactivity toward Human and Rat and has been mentioned in 3+ publications.

Quick Overview for HPSE antibody (Middle Region) (ABIN3042457)

Target: HPSE (Heparanase (HPSE))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPSE antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HPSE Antibody

Binding Specificity: AA 301-331, Middle Region

Purpose: Anti-Heparanase 1/HPSE Antibody Picoband®

Sequence: NGRTATKEDF LNPDVLDIFI SSVQKVFQVV E

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Heparanase 1/HPSE Antibody Picoband® (ABIN3042457). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Heparanase 1, different from the related mouse and rat sequences by eight amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Hulett MD, Freeman C, Hamdorf BJ, Baker RT, Harris MJ, Parish CR (July 1999), "Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis", Nature medicine 5 (7): 803-9. 2. Nakajima M, Irimura T, Nicolson GL. (1988), "Heparanases and tumor metastasis", J. Cell. Biochem. 36 (2): 157-167. 3. Toyoshima, M., Nakajima, M. : Human heparanase: purification, characterization, cloning, and expression. J. Biol. Chem. 274: 24153-24160, 1999. 4. Vlodavsky I, Friedmann Y, Elkin M, Aingorn H, Atzmon R, Ishai-Michaeli R, Bitan M, Pappo O, Peretz T, Michal I, Spector L, Pecker I (July 1999), "Mammalian heparanase: gene cloning, expression and function in tumor progression and metastasis", Nature medicine 5 (7): 793-802. 5. Vlodavsky I, Goldshmidt O, Zcharia E, et al. (2003), "Mammalian heparanase: involvement in cancer metastasis, angiogenesis and normal development.", Semin. Cancer Biol. 12 (2): 121-9.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-HPSE antibody (ABIN3042457)

Tang, Piao, Zhao, Mu, Li, Ma, Song, Wang, Zhao, Zhang: "Expression and correlation of matrix metalloproteinase-9 and heparanase in patients with breast cancer." in: Medical oncology (Northwood, London, England), Vol. 31, Issue 7, pp. 26, (2014) (PubMed).

Huining, Yi, Dihong, Yifeng, Man, Ting, Jingting: "Inhibition of choriocarcinoma by Fe3O4-dextran-anti-β-human chorionic gonadotropin nanoparticles containing antisense oligodeoxynucleotide of heparanase." in: International journal of nanomedicine, Vol. 8, pp. 4371-8, (2014) (PubMed).

Tang, Zhang, Zhao, Wang, Lu, Song, Zhao, Kang, Wang, Xu, Tian: "The expression and clinical significance of microRNA-1258 and heparanase in human breast cancer." in: Clinical biochemistry, Vol. 46, Issue 10-11, pp. 926-32, (2013) (PubMed).

Target Details for HPSE

Target: HPSE (Heparanase (HPSE))

Alternative Name: HPSE

Background:

Synonyms: Heparanase,3.2.1.166,Endo-glucoronidase,Heparanase-1,Hpa1,Heparanase 8 kDa subunit,Heparanase 50 kDa subunit,HPSE,HEP, HPA, HPA1, HPR1, HPSE1, HSE1,

Tissue Specificity: Highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues. Also expressed in hair follicles, specifically in both Henle's and Huxley's layers of inner the root sheath (IRS) at anagen phase. .

Background: Heparanase, also known as HPSE, is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate molecules into shorter chain length oligosaccharides. Heparanase is an endo-beta-D-glucuronidase capable of cleaving heparan sulfate and has been implicated in inflammation and tumor angiogenesis and metastasis. The successful penetration of the endothelial cell layer that lines the interior surface of blood vessels is an important process in the formation of blood borne tumour metastases. Heparan sulfate proteoglycans are major constituents of this layer and it has been shown that increased metastatic potential corresponds with increased heparanase activity for a number of cell lines.

Sequence Similarities: Belongs to the cyclin family. Cyclin AB subfamily.

Molecular Weight: 61 kDa

Gene ID: 10855

UniProt: Q9Y251

Pathways: Glycosaminoglycan Metabolic Process