HRG antibody (AA 19-200)

Catalog No. ABIN3042459

Product Details anti-HRG Antibody

Target: HRG (Histidine-Rich Glycoprotein (HRG))

Binding Specificity: AA 19-200

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-HRG Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HRG Antibody Picoband® (ABIN3042459). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HRG recombinant protein (Position: V19-V200). Human HRG shares 71% and 69% amino acid (aa) sequences identity with mouse and rat HRG, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Hennis BC, Kluft C (Sep 1991). "KpnI RFLP in the human histidine-rich glycoprotein gene". Nucleic Acids Res 19 (15): 4311-4311. 2. "Entrez Gene: HRG histidine-rich glycoprotein" 3. Poon IK, Patel KK, Davis DS, Parish CR, Hulett MD (2011). "Histidine-rich glycoprotein: the Swiss Army knife of mammalian plasma". Blood 117 (7): 2093-101.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HRG

Target: HRG (Histidine-Rich Glycoprotein (HRG))

Alternative Name: HRG (HRG Products)

Background:

Synonyms: Histidine-rich glycoprotein,Histidine-proline-rich glycoprotein,HPRG,HRG,

Tissue Specificity: Expressed in macrophages and in malignant cells. Expressed by the liver and secreted in plasma (at protein level). .

Background: Histidine-rich glycoprotein (HRG) is a protein that in humans is encoded by the HRG gene. It is mapped to 3q27.3. HRG contains two cystatin-like domains and is located in plasma and platelets. The protein can bind heme, dyes and divalent metal ions. It also can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. HRG is thought to have multiple roles as a protein in the human blood, including roles in immunity, angiogenesis and coagulation.

Molecular Weight: 60 kDa

Gene ID: 3273

UniProt: P04196