HSPG2 antibody (AA 524-701)

Catalog No. ABIN3042466

Product Details anti-HSPG2 Antibody

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Binding Specificity: AA 524-701

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband® (ABIN3042466). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HSPG2 recombinant protein (Position: F524-K701). Human HSPG2 shares 86% amino acid (aa) sequence identity with mouse HSPG2.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Arikawa-Hirasawa E, Le AH, Nishino I, Nonaka I, Ho NC, Francomano CA et al. (May 2002). 2. Iozzo RV (1994). "Perlecan: a gem of a proteoglycan". Matrix Biol. 14 (3): 203-8. 3. Kallunki P, Eddy RL, Byers MG, Kestilä M, Shows TB, Tryggvason K (October 1991). "Cloning of human heparan sulfate proteoglycan core protein, assignment of the gene (HSPG2) to 1p36.1----p35 and identification of a BamHI restriction fragment length polymorphism". Genomics 11 (2): 389-96.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Alternative Name: HSPG2 (HSPG2 Products)

Background:

Synonyms: Basement membrane-specific heparan sulfate proteoglycan core protein,HSPG,Perlecan,PLC,Endorepellin,LG3 peptide,HSPG2,

Tissue Specificity: Found in the basement membranes.

Background: Perlecan (PLC) also known as HSPG2, is a protein that in humans is encoded by the HSPG2 gene. It is mapped to 1p36.12. Perlecan is highly conserved across species and the available data indicate that it has evolved from ancient ancestors by gene duplication and exonshuffling. Perlecan is a key component of the vascular extracellular matrix, here it interacts with a variety of other matrix components and helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. Perlecan can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation.

Sequence Similarities: Contains 4 EGF-like domains.

Molecular Weight: 469 kDa

Gene ID: 3339

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism