Thrombospondin 1 antibody (N-Term)

Catalog No. ABIN3042477

Product Details anti-Thrombospondin 1 Antibody

Target: Thrombospondin 1 (THBS1)

Binding Specificity: AA 58-86, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Thrombospondin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Thrombospondin-1(THBS1) detection. Tested with WB in Human.

Sequence: AFRIEDANLI PPVPDDKFQD LVDAVRAEK

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Thrombospondin-1(THBS1) detection. Tested with WB in Human.
Gene Name: thrombospondin 1
Protein Name: Thrombospondin-1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human THBS1 (58-86aa AFRIEDANLIPPVPDDKFQDLVDAVRAEK), different from the related mouse sequence by five amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for THBS1 is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Thrombospondin 1

Target: Thrombospondin 1 (THBS1)

Alternative Name: THBS1 (THBS1 Products)

Background: THBS1 is also known as Thrombospondin 1, TSP1. The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. It is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Also this protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. In addition, the thrombospondin-1 protein is a member of the thrombospondinfamily. It is a multi-domain matrix glycoprotein that has been shown to be a natural inhibitor of neovascularization and tumorigenesis in healthy tissue. Both positive and negative modulation of endothelial cell adhesion, motility, and growth have been attributed to TSP1. This should not be surprising considering that TSP1 interacts with at least 12 cell adhesion receptors, including CD36, αv integrins, β1 integrins, syndecan, and integrin-associated protein (IAP or CD47). It also interacts with numerous proteases involved in angiogenesis, including plasminogen, urokinase, matrix metalloproteinase, thrombin, cathepsin, and elastase.

Synonyms: THBS 1 antibody|THBS antibody|THBS1 antibody|Thrombospondin 1 antibody|Thrombospondin-1 antibody|Tsp 1 antibody|TSP antibody|Tsp1 antibody|TSP1_HUMAN antibody

Gene ID: 7057

UniProt: P07996

Pathways: Autophagy