SP5 antibody (Middle Region)

Catalog No. ABIN3042490

This Rabbit Polyclonal antibody specifically detects SP5 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for SP5 antibody (Middle Region) (ABIN3042490)

Target: SP5 (Sp5 Transcription Factor (SP5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SP5 Antibody

Binding Specificity: AA 246-275, Middle Region

Purpose: Anti-Sp5 Antibody Picoband®

Sequence: DFAQYQSQIA ALLQTKAPLA ATARRCRRCR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Sp5 Antibody Picoband® (ABIN3042490). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Sp5, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Chen Y, Guo Y, Ge X, Itoh H, Watanabe A, Fujiwara T, Kodama T, Aburatani H: Elevated expression and potential roles of human Sp5, a member of Sp transcription factor family, in human cancers. Biochem Biophys Res Commun, 2006 Feb 17. 2. Simmons SO, Horowitz JM: Nkx3.1 binds and negatively regulates the transcriptional activity of Sp-family members in prostate-derived cells. Biochem J, 2006 Jan 1.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SP5

Target: SP5 (Sp5 Transcription Factor (SP5))

Alternative Name: SP5

Background:

Synonyms: Transcription factor Sp5,SP5,

Tissue Specificity: Expressed in brain and lymphoblasts. .

Background: Sp5 is mapped to 2q31.1. It is a member of the Sp family of zinc finger transcription factors. Like other family members, the Sp5 protein contains a Cys2His2 zinc finger DNA binding domain at the C-terminus. Elevated expression of Sp5 has been noted in several human tumors including hepatocellular carcinoma, gastric cancer and colon cancer.

Sequence Similarities: Belongs to the Sp1 C2H2-type zinc-finger protein family.

Molecular Weight: 42 kDa

Gene ID: 389058