SP5 antibody (Middle Region)

Catalog No. ABIN3042490

Product Details anti-SP5 Antibody

Target: SP5 (Sp5 Transcription Factor (SP5))

Binding Specificity: AA 246-275, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-Sp5 Antibody Picoband®

Sequence: DFAQYQSQIA ALLQTKAPLA ATARRCRRCR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Sp5 Antibody Picoband® (ABIN3042490). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Sp5, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Chen Y, Guo Y, Ge X, Itoh H, Watanabe A, Fujiwara T, Kodama T, Aburatani H: Elevated expression and potential roles of human Sp5, a member of Sp transcription factor family, in human cancers. Biochem Biophys Res Commun, 2006 Feb 17. 2. Simmons SO, Horowitz JM: Nkx3.1 binds and negatively regulates the transcriptional activity of Sp-family members in prostate-derived cells. Biochem J, 2006 Jan 1.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SP5

Target: SP5 (Sp5 Transcription Factor (SP5))

Alternative Name: SP5 (SP5 Products)

Background:

Synonyms: Transcription factor Sp5,SP5,

Tissue Specificity: Expressed in brain and lymphoblasts. .

Background: Sp5 is mapped to 2q31.1. It is a member of the Sp family of zinc finger transcription factors. Like other family members, the Sp5 protein contains a Cys2His2 zinc finger DNA binding domain at the C-terminus. Elevated expression of Sp5 has been noted in several human tumors including hepatocellular carcinoma, gastric cancer and colon cancer.

Sequence Similarities: Belongs to the Sp1 C2H2-type zinc-finger protein family.

Molecular Weight: 42 kDa

Gene ID: 389058