ST5 antibody (Middle Region)

Catalog No. ABIN3042548

The Rabbit Polyclonal anti-ST5 antibody has been validated for WB. It is suitable to detect ST5 in samples from Human, Mouse and Rat.

Quick Overview for ST5 antibody (Middle Region) (ABIN3042548)

Target: ST5 (Suppression of Tumorigenicity 5 (ST5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ST5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ST5 Antibody

Binding Specificity: AA 571-586, Middle Region

Purpose: Anti-ST5 Antibody Picoband®

Sequence: KRHSHDDMLL LAQLSL

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ST5 Antibody (ABIN3042548). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ST5, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: ST5 suppression of tumorigenicity 5" 2. Lichy JH, Modi WS, Seuanez HN, Howley PM (Nov 1992). "Identification of a human chromosome 11 gene which is differentially regulated in tumorigenic and nontumorigenic somatic cell hybrids of HeLa cells".Cell Growth Differ 3 (8): 541-8. 3. Trautmann, U., Vikkula, M., Winterpacht, A., FitzPatrick, D. R., Rauch, A. Disruption of ST5 is associated with mental retardation and multiple congenital abnormalities. J. Med. Genet. 47: 91-98, 2010.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ST5

Target: ST5 (Suppression of Tumorigenicity 5 (ST5))

Alternative Name: ST5

Background:

Synonyms: Suppression of tumorigenicity 5 protein,DENN domain-containing protein 2B,HeLa tumor suppression 1,ST5,DENND2B, HTS1,

Tissue Specificity: Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level). .

Background: Suppression of tumorigenicity 5 is a protein that in humans is encoded by the ST5 gene. This gene is mapped to 11p15.4. The protein encoded by this gene contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells. Three alternatively spliced transcript variants of this gene encoding distinct isoforms are identified.

Sequence Similarities: Contains 1 dDENN domain.

Molecular Weight: 126 kDa

UniProt: P78524