ATG5 antibody (Middle Region)

Catalog No. ABIN3042571

This Rabbit Polyclonal antibody specifically detects ATG5 in WB. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 6+ publications.

Quick Overview for ATG5 antibody (Middle Region) (ABIN3042571)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATG5 Antibody

Binding Specificity: AA 82-97, Middle Region

Purpose: Anti-APG5L/ATG5 Antibody Picoband®

Sequence: DRFDQFWAIN RKLMEY

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-APG5L/ATG5 Antibody (ABIN3042571). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human APG5L, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Baumann P, Cech TR (May 2001). "Pot1, the putative telomere end-binding protein in fission yeast and humans". Science 292 (5519): 1171-5. 2. Hammond EM, Brunet CL, Johnson GD, Parkhill J, Milner AE, Brady G, Gregory CD, Grand RJ (May 1998). "Homology between a human apoptosis specific protein and the product of APG5, a gene involved in autophagy in yeast". FEBS Lett 20 (3): 391-5. 3. Singh, R., Kaushik, S., Wang, Y., Xiang, Y., Novak, I., Komatsu, M., Tanaka, K., Cuervo, A. M., Czaja, M. J. Autophagy regulates lipid metabolism. Nature 458: 1131-1135, 2009.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-ATG5 antibody (ABIN3042571)

Lu, Shi, Ma, Wang, Liu, Chuai, Song, Münsterberg, Cao, Yang: "Proper autophagy is indispensable for angiogenesis during chick embryo development." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 13, pp. 1742-54, (2017) (PubMed).

He, Wang, Han, Jin, Li, Wu, Ma, Cheng, Tang, Yang, Liu: "Nrf2 signalling and autophagy are involved in diabetes mellitus-induced defects in the development of mouse placenta." in: Open biology, Vol. 6, Issue 7, (2017) (PubMed).

Shao, Chen, Chen, Zhang, Lin, Cai, Yin, Tao, Liao, Zhao, Mai, He, He, Cui: "Association between genetic polymorphisms in the autophagy-related 5 gene promoter and the risk of sepsis." in: Scientific reports, Vol. 7, Issue 1, pp. 9399, (2017) (PubMed).

Sun, Gao, Meng, Xu, Wang, Gu, Guo, Shao, Yan, Jiang, Zheng: "Antagomirs Targeting MiroRNA-134 Attenuates Epilepsy in Rats through Regulation of Oxidative Stress, Mitochondrial Functions and Autophagy." in: Frontiers in pharmacology, Vol. 8, pp. 524, (2017) (PubMed).

Yang, Sheng, Yang, Li, Qin, Yu, Zhang: "Endocan silencing induces programmed cell death in hepatocarcinoma." in: Oncology letters, Vol. 14, Issue 5, pp. 5333-5339, (2017) (PubMed).

Song, Li, Lin, Cao: "Anti-autophagic and anti-apoptotic effects of memantine in a SH-SY5Y cell model of Alzheimer's disease via mammalian target of rapamycin-dependent and -independent pathways." in: Molecular medicine reports, Vol. 12, Issue 5, pp. 7615-22, (2015) (PubMed).

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5

Background:

Synonyms: Autophagy protein 5,APG5-like,Apoptosis-specific protein,ATG5,APG5L, ASP,

Tissue Specificity: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.

Background: Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is also known as APG5 or ASP, and this gene is mapped to 6q21. It is found that knockdown of ATG5 in hepatocytes increased triglyceride levels with oleate or a second endogenous stimulus for triglyceride formation. These hepatocytes with ATG5 knockdown also had increased lipid droplet number and size. ATG5 is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.

Sequence Similarities: Belongs to the ubiquitin-conjugating enzyme family.

Molecular Weight: 55 kDa

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy