L-Selectin antibody (Middle Region)

Catalog No. ABIN3042616

This Rabbit Polyclonal antibody specifically detects L-Selectin in WB. It exhibits reactivity toward Mouse and Rat.

Quick Overview for L-Selectin antibody (Middle Region) (ABIN3042616)

Target: L-Selectin (SELL) (Selectin L (SELL))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This L-Selectin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-L-Selectin Antibody

Binding Specificity: AA 125-141, Middle Region

Purpose: Anti-CD62L/SELL Antibody Picoband®

Sequence: KEDCVEIYIK RERDSGK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CD62L/SELL Antibody (ABIN3042616). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse L-selectin, identical to the related rat sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
1. "Entrez Gene: SELL selectin L (lymphocyte adhesion molecule 1)". 2. Cotran, Kumar, Collins (1998). Robbins Pathologic Basis of Disease. Philadelphia: W.B Saunders Company.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Alternative Name: SELL

Background:

Synonyms: L-selectin,CD62 antigen-like family member L,Leukocyte adhesion molecule 1,LAM-1,Leukocyte-endothelial cell adhesion molecule 1,LECAM1,Lymph node homing receptor,Lymphocyte antigen 22,Ly-22,Lymphocyte surface MEL-14 antigen,CD62L,Sell,Lnhr, Ly-22, Ly22,

Tissue Specificity: Predominantly expressed in lymphoid tissue. .

Background: L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17. SELL (L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point.

Sequence Similarities: Belongs to the selectin/LECAM family.

Molecular Weight: 70 kDa

UniProt: P18337