IL-18 antibody (C-Term)

Catalog No. ABIN3042624

This anti-IL-18 antibody is a Rabbit Polyclonal antibody detecting IL-18 in WB. Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for IL-18 antibody (C-Term) (ABIN3042624)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-18 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IL-18 Antibody

Binding Specificity: AA 175-193, C-Term

Purpose: Anti-Interleukin-18 IL18 Antibody Picoband®

Sequence: KKEDELGDRS IMFTVQNED

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Interleukin-18 IL18 Antibody (ABIN3042624). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of Human IL18.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Okamura H, Tsutsi H, Komatsu T, Yutsudo M, Hakura A, Tanimoto T, Torigoe K, Okura T, Nukada Y, Hattori K (November 1995). "Cloning of a new cytokine that induces IFN-gamma production by T cells". Nature 378 (6552): 88-91. 2. Nolan KF, Greaves DR, Waldmann H (July 1998). "The human interleukin 18 gene IL18 maps to 11q22.2-q22.3, closely linked to the DRD2 gene locus and distinct from mapped IDDM loci". Genomics 51 (1): 161-3.. 3. "Entrez Gene: IL18 interleukin 18 (interferon-gamma-inducing factor)". 4. Nolan, K. F., Greaves, D. R., Waldmann, H. The human interleukin 18 gene IL18 maps to 11q22.2-q22.3, closely linked to the DRD2 gene locus and distinct from mapped IDDM loci. Genomics 51: 161-163, 1998.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-IL-18 antibody (ABIN3042624)

Si, Zhang, Gao, Chen, Zhao, Cheng: "Effects of IFN-? on IL-18 Expression in Pregnant Rats and Pregnancy Outcomes." in: Asian-Australasian journal of animal sciences, Vol. 26, Issue 10, pp. 1399-405, (2014) (PubMed).

Wang, Zhang, Zhang, Xu, Fang: "Expression and localization of IL-18 in the hypothalamic-pituitary-ovarian axis of non-pregnant, pregnant, and abortive rats." in: Journal of reproductive immunology, Vol. 92, Issue 1-2, pp. 45-53, (2011) (PubMed).

Yang, Shen, Chen, Ge: "Role of interleukin 18 in acute lung inflammation induced by gut ischemia reperfusion." in: World journal of gastroenterology, Vol. 11, Issue 29, pp. 4524-9, (2005) (PubMed).

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: IL18

Background:

Synonyms: Interleukin-18,IL-18,Iboctadekin,Interferon gamma-inducing factor,IFN-gamma-inducing factor,Interleukin-1 gamma,IL-1 gamma,IL18,IGIF, IL1F4,

Tissue Specificity: Most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon. Not detected in the peripheral blood leukocytes.

Background: Interleukin-18 also known as IL18 is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine.IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-gamma (IFN-gamma) or type II interferon that plays an important role in activating the macrophages or other cells.The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. The human interleukin 18 gene IL18 maps to 11q22.2-q22.3, closely linked to the DRD2 gene locus and distinct from mapped IDDM loci.

Sequence Similarities: Belongs to the IL-1 family.

Molecular Weight: 100 kDa

UniProt: Q14116

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome