c-FOS antibody (Middle Region)

Catalog No. ABIN3042631

Product Details anti-c-FOS Antibody

Target: c-FOS (c-Fos) (c-Fos)

Binding Specificity: AA 170-187, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-FOS antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Proto-oncogene c-Fos(FOS) detection. Tested with WB in Human,Mouse,Rat.

Sequence: DQLEDEKSAL QTEIANLL

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Proto-oncogene c-Fos(FOS) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: FBJ murine osteosarcoma viral oncogene homolog
Protein Name: Proto-oncogene c-Fos

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human c-Fos(170-187aa DQLEDEKSALQTEIANLL), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-c-FOS antibody (ABIN3042631)

Zheng, Wu, Gao, Wang: "Tumor suppressive role of miR-569 in lung cancer." in: Oncology letters, Vol. 15, Issue 4, pp. 4087-4092, (2018) (PubMed).

Hou, Xia, Xiao, Wan, Zhao: "Neurotoxicity of intrathecal injections of dexmedetomidine into the rat spinal dorsal horn." in: Neural regeneration research, Vol. 7, Issue 23, pp. 1765-70, (2015) (PubMed).

Qiu, Ding, Cui, Hu, Ding: "The Expression Patterns of c-Fos and c-Jun Induced by Different Frequencies of Electroacupuncture in the Brain." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 343682, (2015) (PubMed).

Liu, Peng, Lin, Luo, Jiang, Mo, Yung: "Effect of rhynchophylline on the expression of p-CREB and sc-Fos in triatum and hippocampal CA1 area of methamphetamine-induced conditioned place preference rats." in: Fitoterapia, Vol. 92, pp. 16-22, (2014) (PubMed).

Zhang, Li, Xu, Mei, Zhang, Murong, Sun, Tong: "Changes of c-fos, malondialdehyde and lactate in brain tissue after global cerebral ischemia under different brain temperatures." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 3, pp. 354-8, (2014) (PubMed).

Zhang, Zhang, Goldstein, Li, Ge, He, Ruiz: "The effect of c-fos on acute myocardial infarction and the significance of metoprolol intervention in a rat model." in: Cell biochemistry and biophysics, Vol. 65, Issue 2, pp. 249-55, (2013) (PubMed).

Li, Liang, Chen, Zhang, Lin, Huang: "L-dopa methyl ester attenuates amblyopia-induced neuronal injury in visual cortex of amblyopic cat." in: Gene, Vol. 527, Issue 1, pp. 115-22, (2013) (PubMed).

Gong, Wang, Liang, Duan, Ouyang, Yu, Xu, Shen, Weng, Wu: "Soluble MOG35-55/I-A(b) dimers ameliorate experimental autoimmune encephalomyelitis by reducing encephalitogenic T cells." in: PLoS ONE, Vol. 7, Issue 10, pp. e47435, (2013) (PubMed).

Peng, Zhang, Ren, Zhang, Wang: "Effect of ketamine administration on memory consolidation, p-CREB and c-fos expression in the hippocampal slices of minor rats." in: Molecular biology reports, Vol. 38, Issue 4, pp. 2401-7, (2011) (PubMed).

Zhang, He, Goldstein, Ge, Wang, Ruiz: "The expression and significance of proto-oncogene c-fos in viral myocarditis." in: Virology journal, Vol. 7, pp. 285, (2010) (PubMed).

Lou, Jiang, Li, Zhou, He, Lei, Li, Jia: "Herb medicine Gan-fu-kang attenuates liver injury in a rat fibrotic model." in: Journal of ethnopharmacology, Vol. 128, Issue 1, pp. 131-8, (2010) (PubMed).

Xu, Tang, Li, Shi, Chen, Liang: "Positional and expressive alteration of prohibitin during the induced differentiation of human hepatocarcinoma SMMC-7721 cells." in: World journal of gastroenterology, Vol. 14, Issue 32, pp. 5008-14, (2008) (PubMed).

Target Details for c-FOS

Target: c-FOS (c-Fos) (c-Fos)

Alternative Name: FOS (c-Fos Products)

Background: The human oncogene c-fos is cellular homolog of the transforming gene of Finkel-Biskis-Jinkins(FBJ) murine osteosarcoma virus which was mapped to a single human chromosome. c-Fos is encoded by the FOS gene. FOS was the first transcription factor identified that has a critical function in regulating the development of cells destined to form and maintain the skeleton. FOS is also a major component of the activator protein-1(AP-1) transcription factor complex, which includes members of the JUN family. c-fos is a major nuclear target for signal transduction pathways involved in the regulation of cell growth, differentiation, and transformation. Using transgenic and knockout mice, Grigoriadis et al.(1995) established a unique role for the proto-oncogene and nuclear transcription factor, Fos, in regulating the differentiation and activity of specific bone cell populations, both during normal development and in bone disease.

Synonyms: Activator protein 1 antibody|AP 1 antibody|Cellular oncogene c fos antibody|Cellular oncogene fos antibody|FBJ murine osteosarcoma viral(v fos) oncogene homolog(oncogene FOS) antibody|FBJ murine osteosarcoma viral v fos oncogene homolog antibody|FBJ Osteosarcoma Virus antibody|FOS antibody|FOS protein antibody|FOS_HUMAN antibody|G0 G1 switch regulatory protein 7 antibody|G0/G1 switch regulatory protein 7 antibody|G0S7 antibody|Oncogene FOS antibody|p55 antibody|proto oncogene c Fos antibody|Proto oncogene protein c fos antibody|Proto-oncogene c-Fos antibody|v fos FBJ murine osteosarcoma viral oncogene homolog antibody

UniProt: P01100

Pathways: S100 Proteins