Mesothelin antibody (C-Term)

Catalog No. ABIN3042647

The Rabbit Polyclonal anti-Mesothelin antibody has been validated for WB. It is suitable to detect Mesothelin in samples from Human and Monkey.

Quick Overview for Mesothelin antibody (C-Term) (ABIN3042647)

Target: Mesothelin (MSLN)

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mesothelin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Mesothelin Antibody

Binding Specificity: AA 275-288, C-Term

Purpose: Anti-G-protein coupled receptor 30/GPER1 Antibody Picoband®

Sequence: ENVFISVHLL QRTQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-G-protein coupled receptor 30/GPER1 Antibody (ABIN3042647). Tested in WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human G-protein coupled receptor 30, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Monkey
1. Pietras, R. J., Levin, E. R., Szego, C. M. Estrogen receptors and cell signaling. (Letter)Science 310: 51-52, 2005. 2. Revankar, C. M., Cimino, D. F., Sklar, L. A., Arterburn, J. B., Prossnitz, E. R. A transmembrane intracellular estrogen receptor mediates rapid cell signaling. Science 307: 1625-1630, 2005. 3. Takada, Y., Kato, C., Kondo, S., Korenaga, R., Ando, J. Cloning of cDNAs encoding G protein-coupled receptor expressed in human endothelial cells exposed to fluid shear stress. Biochem. Biophys. Res. Commun. 240: 737-741, 1997.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: GPER1

Background:

Synonyms: G-protein coupled estrogen receptor 1,Chemoattractant receptor-like 2,Flow-induced endothelial G-protein coupled receptor 1,FEG-1,G protein-coupled estrogen receptor 1,G-protein coupled receptor 30,GPCR-Br,IL8-related receptor DRY12,Lymphocyte-derived G-protein coupled receptor,LYGPR,Membrane estrogen receptor,mER,GPER1,CEPR, CMKRL2, DRY12, GPER, GPR30,

Tissue Specificity: Expressed in placenta, endothelial and epithelial cells, non laboring and laboring term myometrium, fibroblasts and cancer-associated fibroblasts (CAF), prostate cancer cells and invasive adenocarcinoma (at protein level). Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen. .

Background: G-protein coupled estrogen receptor 1 (GPER1), also known as the GPR30 or CEPR is a G protein-coupled receptor that in humans is encode d by the GPER gene. This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. It is mapped to 7p22.3. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.

Sequence Similarities: Belongs to the G-protein coupled receptor 1 family.

Molecular Weight: 60 kDa

UniProt: Q99527

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process