Mip (AA 246-263), (C-Term) antibody

Catalog No. ABIN3042655

This Rabbit Polyclonal antibody specifically detects in WB. It exhibits reactivity toward Human, Rat and Mouse and has been mentioned in 3+ publications.

Quick Overview for Mip (AA 246-263), (C-Term) antibody (ABIN3042655)

Target: Mip

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: AA 246-263, C-Term

Purpose: Anti-Aquaporin 0/MIP Antibody Picoband®

Sequence: NGQPEGTGEP VELKTQAL

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Aquaporin 0/MIP Antibody (ABIN3042655). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Aquaporin 0, identical to the related rat sequence, and different from the related human sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Human, Rat
1. Berry, V., Francis, P., Kaushal, S., Moore, A., Bhattacharya, S. Missense mutations in MIP underlie autosomal dominant 'polymorphic' and lamellar cataracts linked to 12q. Nature Genet. 25: 15-17, 2000. 2. Francis, P., Chung, J.-J., Yasui, M., Berry, V., Moore, A., Wyatt, M. K., Wistow, G., Bhattacharya, S. S., Agre, P. Functional impairment of lens aquaporin in two families with dominantly inherited cataracts. Hum. Molec. Genet. 9: 2329-2334, 2000. 3. Pisano MM, Chepelinsky AB (Mar 1992). "Genomic cloning, complete nucleotide sequence, and structure of the human gene encoding the major intrinsic protein (MIP) of the lens". Genomics 11 (4): 981-90.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References

Liu, An, Liu, Li, Tang, Chen: "Mouse lung slices: An ex vivo model for the evaluation of antiviral and anti-inflammatory agents against influenza viruses." in: Antiviral research, Vol. 120, pp. 101-11, (2015) (PubMed).

Sun, Zhao, Yao, Zhao, Wu, Wang, Pan, Miao, Guo, Lu: "Wogonoside protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting NF-?B and NLRP3 inflammasome activation." in: Biochemical pharmacology, Vol. 94, Issue 2, pp. 142-54, (2015) (PubMed).

Bruns, Cadeddu, Brueckmann, Fröbel, Geyh, Büst, Fischer, Roels, Wilk, Schildberg, Hünerlitürkoglu, Zilkens, Jäger, Steidl, Zohren, Fenk, Kobbe, Brors, Czibere, Schroeder, Trumpp, Haas: "Multiple myeloma-related deregulation of bone marrow-derived CD34(+) hematopoietic stem and progenitor cells." in: Blood, Vol. 120, Issue 13, pp. 2620-30, (2012) (PubMed).

Target Details

Target: Mip

Background:

Synonyms: Lens fiber major intrinsic protein,Aquaporin-0,MIP26,MP26,Mip,

Tissue Specificity: Major component of lens fiber gap junctions.

Background: Lens fiber major intrinsic protein also called MIP26 or MP26 is a protein that in humans is encoded by the MIP gene. MIP is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. Using 2-color fluorescence in situ hybridization on high-resolution R-banded chromosomes and human genomic DNA clones for MIP as probes, this gene was found that located in close proximity in region 12q13. MIP plays a crucial role in the development of a transparent eye lens. This gene may be responsible for regulating the osmolarity of the lens and interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core.

Molecular Weight: 50 kDa

UniProt: P09011