Desmoglein 2 antibody (C-Term)

Catalog No. ABIN3042669

Product Details anti-Desmoglein 2 Antibody

Target: Desmoglein 2 (DSG2)

Binding Specificity: AA 1104-1118, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Desmoglein 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Desmoglein-2(DSG2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: STRVTKHSTV QHSYS

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Desmoglein-2(DSG2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: desmoglein 2
Protein Name: Desmoglein-2

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Desmoglein 2(1104-1118aa STRVTKHSTVQHSYS), different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Desmoglein 2

Target: Desmoglein 2 (DSG2)

Alternative Name: DSG2 (DSG2 Products)

Background: Desmoglein-2 is a protein that in humans is encoded by the DSG2 gene. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in DSG2 display a high degree of penetrance. Disease expression was of variable severity with LV involvement a prominent feature. The low prevalence of classical ECG changes highlights the need to expand current diagnostic criteria to take account of LV disease, childhood disease expression, and incomplete penetrance.

Synonyms: ARVC 10 antibody|ARVC10 antibody|ARVD 10 antibody|ARVD10 antibody|Cadherin family member 5 antibody|CDHF 5 antibody|CDHF5 antibody|CMD1BB antibody|Desmoglein 2 antibody|Desmoglein-2 antibody|Desmoglein2 antibody|DSG 2 antibody|DSG2 antibody|DSG2_HUMAN antibody|HDGC antibody|HDGC included antibody|MGC117034 antibody|MGC117036 antibody|MGC117037 antibody

UniProt: Q14126

Pathways: Cell-Cell Junction Organization