CTH antibody (C-Term)

Catalog No. ABIN3042672

This anti-CTH antibody is a Rabbit Polyclonal antibody detecting CTH in WB, ICC, FACS and IF. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 5+ publications.

Quick Overview for CTH antibody (C-Term) (ABIN3042672)

Target: CTH (Cystathionase (Cystathionine gamma-Lyase) (CTH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTH antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-CTH Antibody

Binding Specificity: AA 316-331, C-Term

Purpose: Anti-Cystathionase/CTH Antibody Picoband®

Sequence: FYIKGTLQHA EIFLKN

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cystathionase/CTH Antibody (ABIN3042672). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cystathionase, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Donald, L. J., Wang, H. S., Hamerton, J. L. Assignment of the gene for cystathionase (CYS) to human chromosome 16. (Abstract) Cytogenet. Cell Genet. 32: 268 only, 1982. 2. Yang, G., Wu, L., Jiang, B., Yang, W., Qi, J., Cao, K., Meng, Q., Mustafa, A. K., Mu, W., Zhang, S., Snyder, S. H., Wang, R. H2S as a physiologic vasorelaxant: hypertension in mice with deletion of cystathionine gamma-lyase. Science 322: 587-590, 2008.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CTH antibody (ABIN3042672)

Liu, Li, Liang, Li, Jiang, Chu, Yang: "Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway." in: International journal of molecular medicine, Vol. 41, Issue 4, pp. 1867-1876, (2018) (PubMed).

Qi, Zhao, Li, Shao, Wang, Sui, Yu, Shao, Gui, Li, Jia, Jiang, Li, Zhang, Wang, Wang: "High C-X-C motif chemokine 5 expression is associated with malignant phenotypes of prostate cancer cells via autocrine and paracrine pathways." in: International journal of oncology, Vol. 53, Issue 1, pp. 358-370, (2018) (PubMed).

Shi, Guo, Shi, Yu, Song, Li: "Caffeic Acid Phenethyl Ester inhibit Hepatic Fibrosis by Nitric Oxide Synthase and Cystathionine Gamma-Lyase in Rats." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 2774-80, (2016) (PubMed).

Chen, Wang, Li, Jiang, Lu, Zhou: "Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats." in: Journal of diabetes research, Vol. 2016, pp. 9726503, (2016) (PubMed).

Guo, Duan, Wang, Zhou, Li, Sun: "Endogenous carbon monoxide downregulates hepatic cystathionine-γ-lyase in rats with liver cirrhosis." in: Experimental and therapeutic medicine, Vol. 10, Issue 6, pp. 2039-2046, (2015) (PubMed).

Target Details for CTH

Target: CTH (Cystathionase (Cystathionine gamma-Lyase) (CTH))

Alternative Name: CTH

Background:

Synonyms: Cystathionine gamma-lyase,4.4.1.1,Cysteine-protein sulfhydrase,Gamma-cystathionase,CTH,

Background: Cystathionine gamma-lyase (or cystathionase) is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate. The International Radiation Hybrid Mapping Consortium mapped the CTH gene to chromosome 1. The CTH gene had earlier been assigned to chromosome 16 by study of somatic cell hybrids. It is demonstrated that hydrogen sulfide (H2S) is physiologically generated by CTH.

Sequence Similarities: Contains 1 bHLH (basic helix-loop-helix) domain.

Molecular Weight: 45 kDa

UniProt: P32929

Pathways: ER-Nucleus Signaling, Warburg Effect