ANGPTL4 antibody (C-Term)

Catalog No. ABIN3042686

The Rabbit Polyclonal anti-ANGPTL4 antibody has been validated for WB. It is suitable to detect ANGPTL4 in samples from Human and Rat.

Quick Overview for ANGPTL4 antibody (C-Term) (ABIN3042686)

Target: ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANGPTL4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ANGPTL4 Antibody

Binding Specificity: AA 349-367, C-Term

Purpose: Anti-Angiopoietin-like 4/ANGPTL4 Antibody Picoband®

Sequence: WWFGTCSHSN LNGQYFRSI

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Angiopoietin-like 4/ANGPTL4 Antibody (ABIN3042686). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Angiopoietin-like 4, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Romeo, S., Pennacchio, L. A., Fu, Y., Boerwinkle, E., Tybjaerg-Hansen, A., Hobbs, H. H., Cohen, J. C.Population-based resequencing of ANGPTL4 uncovers variations that reduce triglycerides and increase HDL. Nature Genet. 39: 513-516, 2007. 2. Yoon, J. C., Chickering, T. W., Rosen, E. D., Dussault, B., Qin, Y., Soukas, A., Friedman, J. M., Holmes, W. E., Spiegelman, B. M.Peroxisome proliferator-activated receptor gamma target gene encoding a novel angiopoietin-related protein associated with adipose differentiation. Molec. Cell. Biol. 20: 5343-5349, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ANGPTL4

Target: ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))

Alternative Name: ANGPTL4

Background:

Synonyms: Angiopoietin-related protein 4,Angiopoietin-like protein 4,Hepatic fibrinogen/angiopoietin-related protein,HFARP,ANGPTL4,ARP4, HFARP, PGAR,PP1158, PSEC0166, UNQ171/PRO197,

Tissue Specificity: Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. .

Background: Angiopoietin-related protein 4 (Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.

Sequence Similarities: Contains 1 fibrinogen C-terminal domain.

Molecular Weight: 45 kDa

Pathways: Regulation of Lipid Metabolism by PPARalpha