BCL2L2 antibody (Middle Region)

Catalog No. ABIN3042693

Product Details anti-BCL2L2 Antibody

Target: BCL2L2 (BCL2-Like 2 (BCL2L2))

Binding Specificity: AA 74-88, Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL2L2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose: Anti-Bcl-2-like protein 2 Bcl2L2 Antibody Picoband®

Sequence: SAQQRFTQVS DELFQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Bcl-2-like protein 2 Bcl2L2 Antibody (ABIN3042693). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human BCL2L2, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Gibson L, Holmgreen SP, Huang DC, Bernard O, Copeland NG, Jenkins NA, Sutherland GR, Baker E, Adams JM, Cory S (Oct 1996). "bcl-w, a novel member of the bcl-2 family, promotes cell survival". Oncogene 13 (4): 665-75. PMID 8761287. 2. "Entrez Gene: BCL2L2 BCL2-like 2".

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BCL2L2

Target: BCL2L2 (BCL2-Like 2 (BCL2L2))

Alternative Name: BCL2L2 (BCL2L2 Products)

Background:

Synonyms: Bcl-2-like protein 2,Bcl2-L-2,Apoptosis regulator Bcl-W,BCL2L2,BCLW, KIAA0271,

Tissue Specificity: Expressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines. .

Background: Bcl-2-like protein 2 is a protein that in humans is encoded by the BCL2L2 gene. This gene encodes a pro-survival (anti-apoptotic) member of the bcl-2 protein family. Its gene is mapped to human chromosome 14q11.2-q12. In all, it has got 193 amino acids. Generally speaking, the BCL2 can be found in brain, colon and salivary gland. Acting as the anti- and pro-apoptotic regulators, it has played a vital role in reducing the cell apoptosis under cytotoxic conditions.

Sequence Similarities: Belongs to the Bcl-2 family.

Molecular Weight: 21 kDa

UniProt: Q92843