Podoplanin antibody (C-Term)

Catalog No. ABIN3042706

This Rabbit Polyclonal antibody specifically detects Podoplanin in WB. It exhibits reactivity toward Human.

Quick Overview for Podoplanin antibody (C-Term) (ABIN3042706)

Target: Podoplanin (PDPN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Podoplanin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Podoplanin Antibody

Binding Specificity: AA 226-238, C-Term

Purpose: Anti-Podoplanin/gp36/PDPN Antibody Picoband®

Sequence: VVVMRKMSGR YSP

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Podoplanin/gp36/PDPN Antibody (ABIN3042706). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Podoplanin/gp36.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Zimmer G, Oeffner F, Von Messling V, Tschernig T, Groness HJ, Klenk HD, Herrler G (Sep 1999). "Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium". Biochem J 341 ( Pt 2): 277-84. PMC 1220357. PMID 10393083. 2. Ma T, Yang B, Matthay MA, Verkman AS (Jul 1998). "Evidence against a role of mouse, rat, and two cloned human t1alpha isoforms as a water channel or a regulator of aquaporin-type water channels". Am J Respir Cell Mol Biol 19 (1): 143-9. PMID 9651190. 3. Entrez Gene: PDPN podoplanin".

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: PDPN

Background:

Synonyms: Podoplanin,Aggrus,Glycoprotein 36,Gp36,PA2.26 antigen,T1-alpha,T1A,PDPN ,GP36 ,PSEC0003, PSEC0025,

Tissue Specificity: Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25 % of early oral squamous cell carcinomas. .

Background: Podoplanin (PDPN) is a protein that in humans is encoded by the PDPN gene. This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The International radiation Hybrid mapping consortium mapped the T1A gene to chromosome 1. It has got 162 amino acid protein which is a typical type I membrane glycoprotein, with an N-terminal signal sequence, a signal sequence cleavage site, a C-terminal hydrophobic stretch, and a short cytoplasmic tail. Northern blot analysis detected a 1.2-kb T1A2 transcript only in lung. It has been described as a differentiation antigen and influenza-virus receptor, and a marker of lung injury.

Sequence Similarities: Belongs to the podoplanin family.

Molecular Weight: 36 kDa

Pathways: Dicarboxylic Acid Transport