CX3CL1 antibody (N-Term)

Catalog No. ABIN3042714

The Rabbit Polyclonal anti-CX3CL1 antibody (ABIN3042714) specifically detects CX3CL1 in WB and IHC. The antibody is reactive with Rat and Mouse samples.

Quick Overview for CX3CL1 antibody (N-Term) (ABIN3042714)

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CX3CL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CX3CL1 Antibody

Binding Specificity: AA 74-92, N-Term

Purpose: Anti-Fractalkine CX3CL1 Antibody

Sequence: CADPKEKWVQ DAMKHLDHQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Fractalkine CX3CL1 Antibody (ABIN3042714). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse CX3CL1, identical to the related rat sequence.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat, -
Western blot, 0.1-0.5 μg/mL, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CX3CL1

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Alternative Name: CX3CL1

Background:

Synonyms: Fractalkine,C-X3-C motif chemokine 1,CX3C membrane-anchored chemokine,Neurotactin,Small-inducible cytokine D1,Processed fractalkine,Cx3cl1,Cx3c, Fkn, Scyd1,

Tissue Specificity: Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis.

Background: Chemokine (C-X3-C motif) ligand 1, also known as CX3CL1, is a large cytokine protein. It is said to be the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). Its gene is mapped to the long arm of chromosome 8. Being the large protein, it has got 373 amino acids. Imai et al. (1997) identified a 7-transmembrane high-affinity receptor (CX3CR1, 601470) for fractalkine and showed that it mediates both the adhesive and migratory functions of fractalkine.

Molecular Weight: 90-100 kDa

UniProt: O35188

Pathways: Synaptic Membrane