HSD17B6 antibody (C-Term)

Catalog No. ABIN3042725

The Rabbit Polyclonal anti-HSD17B6 antibody has been validated for WB and IHC. It is suitable to detect HSD17B6 in samples from Human.

Quick Overview for HSD17B6 antibody (C-Term) (ABIN3042725)

Target: HSD17B6 (Hydroxysteroid (17-Beta) Dehydrogenase 6 (HSD17B6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HSD17B6 Antibody

Binding Specificity: AA 300-317, C-Term

Purpose: Anti-HSD17B6 Antibody Picoband®

Sequence: SLADYILTRS WPKPAQAV

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HSD17B6 Antibody (ABIN3042725). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HSD17B6.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Baker ME (Feb 2001). "Evolution of 17beta-hydroxysteroid dehydrogenases and their role in androgen, estrogen and retinoid action". Mol Cell Endocrinol 171 (1-2): 211-5. doi:10.1016/S0303-7207(00)00414-7. PMID 11165032. 2. Biswas, M. G., Russell, D. W. Expression cloning and characterization of oxidative 17-beta- and 3-alpha -hydroxysteroid dehydrogenases from rat and human prostate. J. Biol. Chem. 272: 15959-15966, 1997.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HSD17B6

Target: HSD17B6 (Hydroxysteroid (17-Beta) Dehydrogenase 6 (HSD17B6))

Alternative Name: HSD17B6

Background:

Synonyms: 17-beta-hydroxysteroid dehydrogenase type 6,17-beta-HSD 6,17-beta-HSD6,1.1.1.105,1.1.1.239,1.1.1.62,3-alpha->beta-hydroxysteroid epimerase,3-alpha->beta-HSE,Oxidative 3-alpha hydroxysteroid dehydrogenase,Short chain dehydrogenase/reductase family 9C member 6,HSD17B6,RODH, SDR9C6,

Tissue Specificity: Detected in liver and prostate (at protein level). Detected in adult liver, lung, brain, placenta, prostate, adrenal gland, testis, mammary gland, spleen, spinal cord and uterus. Detected in caudate nucleus, and at lower levels in amygdala, corpus callosum, hippocampus, substantia nigra and thalamus. Detected in fetal lung, liver and brain. .

Background: Hydroxysteroid 17-beta dehydrogenase 6 is an enzyme that in humans is encoded by the HSD17B6 gene. The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. Baker ME et al point out expression of 17beta-HSDs had an important role in the early evolution of the physiological response to androgens and estrogens. Biswas and Russell concluded that 17beta-HSD6 and RoDH play opposing roles in androgen action, 17beta-HSD6 inactivates 3alpha-adiol by conversion to androsterone and RoDH activates 3alpha-adiol by conversion to dihydrotestosterone. The synthesis of an active steroid hormone by back conversion of an inactive metabolite represents a potentially important mechanism by which the steady state level of a transcriptional effector can be regulated.

Sequence Similarities: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Molecular Weight: 35 kDa

UniProt: O14756

Pathways: Steroid Hormone Biosynthesis