Claudin 2 antibody (AA 38-230)

Catalog No. ABIN3042751

Product Details anti-Claudin 2 Antibody

Target: Claudin 2 (CLDN2)

Binding Specificity: AA 38-230

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-Claudin 2/CLDN2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Claudin 2/CLDN2 Antibody (ABIN3042751). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Claudin 2 recombinant protein (Position: A38-V230). Human Claudin 2 shares 92% amino acid (aa) sequence identity with mouse Claudin 2.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Rat, Human
1. Morita K, Furuse M, Fujimoto K, Tsukita S (Mar 1999). "Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands". Proc Natl Acad Sci U S A 96 (2): 511-6. 2. Sakaguchi, T., Gu, X., Golden, H. M., Suh, E., Rhoads, D. B., Reinecker, H.-C. Cloning of the human claudin-2 5-prime-flanking region revealed a TATA-less promoter with conserved binding sites in mouse and human for caudal-related homeodomain proteins and hepatocyte nuclear factor-1-alpha. J. Biol. Chem. 277: 21361-21370, 2002. 3. Muto, S., Hata, M., Taniguchi, J., Tsuruoka, S., Moriwaki, K., Saitou, M., Furuse, K., Sasaki, H., Fujimura, A., Imai, M., Kusano, E., Tsukita, S., Furuse, M. (2010). "Claudin-2-deficient mice are defective in the leaky and cation-selective paracellular permeability properties of renal proximal tubules". Proceedings of the National Academy of Sciences 107 (17): 8011-8016.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Claudin 2 antibody (ABIN3042751)

Cen, Liu, Li, He, Wang, Liu, Liu, Ji: "Alteration in P-glycoprotein at the blood-brain barrier in the early period of MCAO in rats." in: The Journal of pharmacy and pharmacology, Vol. 65, Issue 5, pp. 665-72, (2013) (PubMed).

Target Details for Claudin 2

Target: Claudin 2 (CLDN2)

Alternative Name: CLDN2 (CLDN2 Products)

Background:

Synonyms: Claudin-2,SP82,CLDN2,PSEC0059, SP82, UNQ705/PRO1356,

Background: Claudin-2 is a protein that in humans is encoded by the CLDN2 gene. By genomic sequence analysis, this gene is mapped to chromosome Xq22.3-q23. This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. By expression in a human intestinal epithelial cell line, it was determined that the intestine-specific homeodomain proteins CDX1 and CDX2 activated a reporter plasmid driven by the CLDN2 promoter.

Molecular Weight: 25 kDa

Gene ID: 9075

UniProt: P57739

Pathways: Hepatitis C