CRY2 antibody (N-Term)

Catalog No. ABIN3042760

This Rabbit Polyclonal antibody specifically detects CRY2 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CRY2 antibody (N-Term) (ABIN3042760)

Target: CRY2 (Cryptochrome 2 (Photolyase-Like) (CRY2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRY2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CRY2 Antibody

Binding Specificity: AA 171-200, N-Term

Purpose: Anti-CRY2 Antibody Picoband®

Sequence: RFQAIISRME LPKKPVGLVT SQQMESCRAE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CRY2 Antibody Picoband® (ABIN3042760). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CRY2, different from the related mouse and rat sequences by five amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Kovanen L, Kaunisto M, Donner K, Saarikoski ST, Partonen T. ""CRY2 genetic variants associate with dysthymia."" PLoS One, 2013. 2. Mao Y, Fu A, Hoffman AE, Jacobs DI, Jin M, Chen K, Zhu Y. ""The circadian gene CRY2 is associated with breast cancer aggressiveness possibly via epigenomic modifications."" Tumour Biol, 2015 May.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CRY2

Target: CRY2 (Cryptochrome 2 (Photolyase-Like) (CRY2))

Alternative Name: CRY2

Background:

Synonyms: Cryptochrome-2,CRY2,KIAA0658,

Tissue Specificity: Expressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle. .

Background: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. And it is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene.

Sequence Similarities: Belongs to the DNA photolyase class-1 family.

Molecular Weight: 67 kDa

Gene ID: 1408

Pathways: Response to Water Deprivation, Protein targeting to Nucleus