CRY1 antibody (N-Term)

Catalog No. ABIN3042761

The Rabbit Polyclonal anti-CRY1 antibody has been validated for WB and IHC. It is suitable to detect CRY1 in samples from Human, Mouse and Rat.

Quick Overview for CRY1 antibody (N-Term) (ABIN3042761)

Target: CRY1 (Cryptochrome 1 (Photolyase-Like) (CRY1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRY1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CRY1 Antibody

Binding Specificity: AA 153-189, N-Term

Purpose: Anti-Cryptochrome I/CRY1 Antibody Picoband®

Sequence: FQTLISKMEP LEIPVETITS EVIEKCTTPL SDDHDEK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cryptochrome I/CRY1 Antibody Picoband® (ABIN3042761). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Cryptochrome I, different from the related mouse sequence by seven amino acids, and from the related rat sequence by six amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat, Human1. Griffin EA, Staknis D, Weitz CJ (October 1999). "Light-independent role of CRY1 and CRY2 in the mammalian circadian clock". Science 286 (5440): 768-71. 2. Ukai-Tadenuma M, Yamada RG, Xu H, Ripperger JA, Liu AC, Ueda HR (Jan 2011). "Delay in feedback repression by cryptochrome 1 is required for circadian clock function". Cell 144 (2): 268-81.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CRY1

Target: CRY1 (Cryptochrome 1 (Photolyase-Like) (CRY1))

Alternative Name: CRY1

Background:

Synonyms: Cryptochrome-1,CRY1,PHLL1,

Tissue Specificity: Brain, liver, placenta, lymphocytes and erythrocytes.

Background: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. And this gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of the related gene in mouse results in a shortened circadian cycle in complete darkness.

Sequence Similarities: Belongs to the DNA photolyase class-1 family.

Molecular Weight: 66 kDa

Gene ID: 1407

UniProt: Q16526

Pathways: Response to Water Deprivation, Proton Transport