CTGF antibody (AA 58-349)

Catalog No. ABIN3042771

Product Details anti-CTGF Antibody

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Binding Specificity: AA 58-349

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-CTGF Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CTGF Antibody Picoband® (ABIN3042771). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CTGF recombinant protein (Position: R58-A349). Human CTGF shares 95.5% and 95.9% amino acid (aa) sequence identity with mouse and rat CTGF, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Chen CC, Lau LF (April 2009). "Functions and mechanisms of action of CCN matricellular proteins". Int. J. Biochem. Cell Biol. 41 (4): 771-83. 2. Hall-Glenn F, Lyons KM (October 2011). "Roles for CCN2 in normal physiological processes". Cell. Mol. Life Sci. 68 (19): 3209-17. 3. Jun JI, Lau LF (December 2011). "Taking aim at the extracellular matrix: CCN proteins as emerging therapeutic targets". Nat Rev Drug Discov 10 (12): 945-63.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-CTGF antibody (ABIN3042771)

Lu, Kang, Hu, Tang, Zhou, Yu, Xu: "Angiotensin-(1-7) relieved renal injury induced by chronic intermittent hypoxia in rats by reducing inflammation, oxidative stress and fibrosis." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, Vol. 50, Issue 1, pp. e5594, (2017) (PubMed).

Lv, Wu, Li, Wang, Wang, Zhang, Xu, Zhang: "Effect of QiShenYiQi pill on myocardial collagen metabolism in experimental autoimmune myocarditis rats." in: Biomedicine & pharmacotherapy, Vol. 88, pp. 894-901, (2017) (PubMed).

Zhang, Li, Zheng, Lin, Zhang, Zhang: "Inhibition of angiogenesis by arsenic trioxide via TSP-1-TGF-β1-CTGF-VEGF functional module in rheumatoid arthritis." in: Oncotarget, Vol. 8, Issue 43, pp. 73529-73546, (2017) (PubMed).

Yang, Luo, Zhu, Zhou, Wang, Xue, Zhang, Cai, Chen, Ma, Chen, Wang, Luo, Liu, Zhao: "Chlorogenic Acid Inhibits Liver Fibrosis by Blocking the miR-21-Regulated TGF-β1/Smad7 Signaling Pathway in Vitro and in Vivo." in: Frontiers in pharmacology, Vol. 8, pp. 929, (2017) (PubMed).

Bai, Li, Zhao, Liu, Wang, Lian, Zhao, Wang: "PCPA protects against monocrotaline-induced pulmonary arterial remodeling in rats: potential roles of connective tissue growth factor." in: Oncotarget, Vol. 8, Issue 67, pp. 111642-111655, (2017) (PubMed).

Cheng, Xiao, Ainiwaer, Wang, Wu, Mao, Yang, Bao: "Molecular responses of radiation-induced liver damage in rats." in: Molecular medicine reports, Vol. 11, Issue 4, pp. 2592-600, (2015) (PubMed).

Ma, Li, Yue, Guo, Xu, Xu, Jia, Yu, Zhang, Liu, Liu, Shao, Chen, Liu: "Cryptotanshinone attenuates cardiac fibrosis via downregulation of COX-2, NOX-2, and NOX-4." in: Journal of cardiovascular pharmacology, Vol. 64, Issue 1, pp. 28-37, (2014) (PubMed).

Jin, Shi, Zou, Miao, Sun, Li: "Fenugreek Prevents the Development of STZ-Induced Diabetic Nephropathy in a Rat Model of Diabetes." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 259368, (2014) (PubMed).

Wang, Zhuang, Gao, Zhang, Yang: "Increased expression of CCN2 in the red flashing light-induced myopia in guinea pigs." in: BioMed research international, Vol. 2013, pp. 761823, (2014) (PubMed).

Guo, Qi, Yu, Du, Wu, Liu: "Effect of exenatide on the cardiac expression of adiponectin receptor 1 and NADPH oxidase subunits and heart function in streptozotocin-induced diabetic rats." in: Diabetology & metabolic syndrome, Vol. 6, Issue 1, pp. 29, (2014) (PubMed).

Li, Dai, Zhao, Zhang, Hui, Zhang, Jin: "Preventative effect of Astragalus flavescens on hepatic fibrosis in rats and its mechanism of action." in: Experimental and therapeutic medicine, Vol. 6, Issue 4, pp. 904-908, (2013) (PubMed).

Wang, Li, Lu, Zhao, Xu: "Taurine attenuates oxidative stress and alleviates cardiac failure in type I diabetic rats." in: Croatian medical journal, Vol. 54, Issue 2, pp. 171-9, (2013) (PubMed).

Wen, Huang, Zhang, Zhang, Chen, Gu, Hao: "Resveratrol attenuates diabetic nephropathy via modulating angiogenesis." in: PLoS ONE, Vol. 8, Issue 12, pp. e82336, (2013) (PubMed).

Li, Zhang, Wang, Zheng, Chen: "Nicousamide blocks the effects of advanced glycation end products on renal cells." in: European journal of pharmacology, Vol. 674, Issue 2-3, pp. 455-9, (2012) (PubMed).

Wang, Li, Lu, Zhao: "Riboflavin alleviates cardiac failure in Type I diabetic cardiomyopathy." in: Heart international, Vol. 6, Issue 2, pp. e21, (2012) (PubMed).

Wang, Hui, Fan, Xiong, Sun: "Filtering bleb area and intraocular pressure following subconjunctival injection of CTGF antibody after glaucoma filtration surgery in rabbits." in: International journal of ophthalmology, Vol. 4, Issue 5, pp. 480-3, (2012) (PubMed).

Wang, Li, Lu, Bao, Zhao: "Luteolin ameliorates cardiac failure in type I diabetic cardiomyopathy." in: Journal of diabetes and its complications, Vol. 26, Issue 4, pp. 259-65, (2012) (PubMed).

Guo, Zhang, Li, Xu: "Effect of telmisartan on the expression of adiponectin receptors and nicotinamide adenine dinucleotide phosphate oxidase in the heart and aorta in type 2 diabetic rats." in: Cardiovascular diabetology, Vol. 11, pp. 94, (2012) (PubMed).

Target Details for CTGF

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Alternative Name: CTGF (CTGF Products)

Background:

Synonyms: Connective tissue growth factor,CCN family member 2,Hypertrophic chondrocyte-specific protein 24,Insulin-like growth factor-binding protein 8,IBP-8,IGF-binding protein 8,IGFBP-8,CTGF,CCN2, HCS24, IGFBP8,

Tissue Specificity: Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested. .

Background: CTGF, also known as CCN2 or connective tissue growth factor, is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins (see also CCN intercellular signaling protein). CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. And the encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.

Sequence Similarities: Belongs to the CCN family.

Molecular Weight: 38 kDa

Gene ID: 1490

UniProt: P29279

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding