Cathepsin B antibody (AA 80-333)

Catalog No. ABIN3042773

The Rabbit Polyclonal anti-Cathepsin B antibody has been validated for WB. It is suitable to detect Cathepsin B in samples from Human. There are 3+ publications available.

Quick Overview for Cathepsin B antibody (AA 80-333) (ABIN3042773)

Target: Cathepsin B (CTSB)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cathepsin B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Cathepsin B Antibody

Binding Specificity: AA 80-333

Purpose: Anti-Cathepsin B/CTSB Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cathepsin B/CTSB Antibody Picoband® (ABIN3042773). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Cathepsin B recombinant protein (Position: L80-D333). Human Cathepsin B shares 83% and 84% amino acid (aa) sequences identity with mouse and rat Cathepsin B, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Chan SJ, San Segundo B, McCormick MB, Steiner DF (October 1986). "Nucleotide and predicted amino acid sequences of cloned human and mouse preprocathepsin B cDNAs". Proc. Natl. Acad. Sci. U.S.A. 83 (20): 7721-5. 2. Cao L, Taggart RT, Berquin IM, Moin K, Fong D, Sloane BF (February 1994). "Human gastric adenocarcinoma cathepsin B: isolation and sequencing of full-length cDNAs and polymorphisms of the gene". Gene 139 (2): 163-9. 3. Wang, X., Chan, S. J., Eddy, R. L., Byers, M. G., Fukushima, Y., Henry, W. M., Haley, L. L., Steiner, D. F., Shows, T. B. : Chromosome assignment of cathepsin B (CTSB) to 8p22 and cathepsin H (CTSH) to 15q24-q25. (Abstract) Cytogenet. Cell Genet. 46: 710-711, 1987. 4. Mueller-Steiner S, Zhou Y, Arai H, Roberson ED, Sun B, Chen J, Wang X, Yu G, Esposito L, Mucke L, Gan L (September 2006). "Antiamyloidogenic and neuroprotective functions of cathepsin B: implications for Alzheimer's disease". Neuron 51 (6): 703-14.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Cathepsin B antibody (ABIN3042773)

Zheng, Li, Wang, Wang, Liao, Hu, Fan, Kang, Zeng, Wu, Wu, Zhang, Wang, He et al.: "Inhibition of autophagosome-lysosome fusion by ginsenoside Ro via the ESR2-NCF1-ROS pathway sensitizes esophageal cancer cells to 5-fluorouracil-induced cell death via the CHEK1-mediated DNA damage ..." in: Autophagy, Vol. 12, Issue 9, pp. 1593-613, (2017) (PubMed).

Zhang, Mai, Yang, Dong, Zheng, Yang: "CTSB promotes porcine preadipocytes differentiation by degrading fibronectin and attenuating the Wnt/β-catenin signaling pathway." in: Molecular and cellular biochemistry, Vol. 395, Issue 1-2, pp. 53-64, (2014) (PubMed).

Yang, Lü, Hong, Xi, Zhang: "The molecular mechanism for effects of TiN coating on NiTi alloy on endothelial cell function." in: Biomaterials, Vol. 35, Issue 24, pp. 6195-205, (2014) (PubMed).

Target Details for Cathepsin B

Target: Cathepsin B (CTSB)

Alternative Name: CTSB

Background:

Synonyms: Cathepsin B,3.4.22.1,APP secretase,APPS,Cathepsin B1,Cathepsin B light chain,Cathepsin B heavy chain,CTSB,CPSB,

Tissue Specificity: Expressed in all cell types so far analyzed.

Background: < a href="https://www.bosterbio.com/anti-cathepsin-b-picoband-trade-antibody-pb9046-boster.html" style="color:#ea8d28">Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene. And this gene is mapped to chromosome 8p22. The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

Sequence Similarities: Belongs to the peptidase C1 family.

Molecular Weight: 33 kDa

Gene ID: 1508

UniProt: P07858

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades