Cullin 1 antibody (AA 457-770)

Catalog No. ABIN3042777

This anti-Cullin 1 antibody is a Rabbit Polyclonal antibody detecting Cullin 1 in WB. Suitable for Human, Mouse and Rat.

Quick Overview for Cullin 1 antibody (AA 457-770) (ABIN3042777)

Target: Cullin 1 (CUL1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Cullin 1 Antibody

Binding Specificity: AA 457-770

Purpose: Anti-Cullin 1/CUL1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cullin 1/CUL1 Antibody Picoband® (ABIN3042777). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Cullin 1 recombinant protein (Position: E457-D770). Human Cullin 1 shares 100% amino acid (aa) sequence identity with mouse Cullin 1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Kipreos ET, Lander LE, Wing JP, et al. (1996). "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family.". Cell 85 (6): 829-39. Bonaldo MF, Lennon G, Soares MB (1997). 2. Michel, J. J., Xiong, Y. Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9: 435-449, 1998. 3. "Normalization and subtraction: two approaches to facilitate gene discovery.". Genome Res. 6 (9): 791-806.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Cullin 1

Target: Cullin 1 (CUL1)

Alternative Name: CUL1

Background:

Synonyms: Cullin-1,CUL-1,CUL1,

Tissue Specificity: Expressed in lung fibroblasts. .

Background: Cullin 1, also known as CUL1, is a human protein and gene from cullin family. This protein plays an important role in protein degradation and protein ubiquitination. This is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Human cullin 1, but not the other closely related cullins 2, 3, 4A, and 5, selectively interacts with human SKP1.

Sequence Similarities: Belongs to the CCN family.

Molecular Weight: 90 kDa

UniProt: Q13616

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases, Regulation of Cell Size