CYP24A1 antibody (AA 153-514)

Catalog No. ABIN3042784

This Rabbit Polyclonal antibody specifically detects CYP24A1 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CYP24A1 antibody (AA 153-514) (ABIN3042784)

Target: CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Polypeptide 1 (CYP24A1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP24A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CYP24A1 Antibody

Binding Specificity: AA 153-514

Purpose: Anti-CYP24A1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CYP24A1 Antibody Picoband® (ABIN3042784). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CYP24A1 recombinant protein (Position: E153-R514). Human CYP24A1 shares 86.2% amino acid (aa) sequence identity with both mouse and rat CYP24A1.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: CYP24A1 cytochrome P450, family 24, subfamily A, polypeptide 1". 2. Dauber A, Nguyen TT, Sochett E, Cole DE, Horst R, Abrams SA, Carpenter TO, Hirschhorn JN (February 2012). "Genetic defect in CYP24A1, the vitamin D 24-hydroxylase gene, in a patient with severe infantile hypercalcemia". J. Clin. Endocrinol. Metab. 97 (2): E268-74. 3. Sakaki T, Kagawa N, Yamamoto K, Inouye K (January 2005). "Metabolism of vitamin D3 by cytochromes P450". Front. Biosci. 10: 119-34.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CYP24A1

Target: CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Polypeptide 1 (CYP24A1))

Alternative Name: CYP24A1

Background:

Synonyms: 1,25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial,24-OHase,Vitamin D (3) 24-hydroxylase,1.14.13.126,Cytochrome P450 24A1,Cytochrome P450-CC24,CYP24A1,CYP24,

Tissue Specificity: Expressed in many tissues. .

Background: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. And this mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Sequence Similarities: Belongs to the cytochrome P450 family.

Molecular Weight: 59 kDa

Gene ID: 1591

UniProt: Q07973

Pathways: Metabolism of Steroid Hormones and Vitamin D