CYP24A1 antibody (C-Term)

Catalog No. ABIN6258419

This Rabbit Polyclonal antibody specifically detects CYP24A1 in WB, ELISA, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for CYP24A1 antibody (C-Term) (ABIN6258419)

Target: CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Polypeptide 1 (CYP24A1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP24A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-CYP24A1 Antibody

Binding Specificity: C-Term

Specificity: Cytochrome P450 24A1 Antibody detects endogenous levels of total Cytochrome P450 24A1.

Predicted Reactivity: Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Cytochrome P450 24A1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CYP24A1

Target: CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Polypeptide 1 (CYP24A1))

Alternative Name: CYP24A1

Background:

Description: Has a role in maintaining calcium homeostasis. Catalyzes the adrenodoxin-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and calcitriol (1-alpha,25-dihydroxyvitamin D3). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D3-26,23-lactone as end product.

Gene: CYP24A1

Molecular Weight: 60 kDa

Gene ID: 1591

UniProt: Q07973

Pathways: Metabolism of Steroid Hormones and Vitamin D