CD86 antibody (AA 24-329)

Catalog No. ABIN3042788

The Rabbit Polyclonal anti-CD86 antibody has been validated for WB and IHC. It is suitable to detect CD86 in samples from Human and Mouse.

Quick Overview for CD86 antibody (AA 24-329) (ABIN3042788)

Target: CD86

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD86 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CD86 Antibody

Binding Specificity: AA 24-329

Purpose: Anti-CD86 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CD86 Antibody Picoband® (ABIN3042788). Tested in IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD86 recombinant protein (Position: A24-F329). Human CD86 shares 50% amino acid (aa) sequence identity with mouse CD86.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL
1. Chen C, Gault A, Shen L, Nabavi N (May 1994). "Molecular cloning and expression of early T cell costimulatory molecule-1 and its characterization as B7-2 Molecule". J. Immunol. 152 (10): 4929-36. 2. Reeves, R. H., Patch, D., Sharpe, A. H., Borriello, F., Freeman, G. J., Edelhoff, S., Disteche, C. The costimulatory genes Cd80 and Cd86 are linked on mouse chromosome 16 and human chromosome 3. Mammalian Genome 8: 581-582, 1997. 3. Shah, R., Banks, K., Patel, A., Dogra, S., Terrell, R., Powers, P. A., Fenton, C., Dinauer, C. A., Tuttle, R. M., Francis, G. L. Intense expression of the B7-2 antigen presentation coactivator is an unfavorable prognostic indicator for differentiated thyroid carcinoma of children and adolescents. J. Clin. Endocr. Metab. 87: 4391-4397, 2002.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background:

Synonyms: T-lymphocyte activation antigen CD86,Activation B7-2 antigen,B70,BU63,CTLA-4 counter-receptor B7.2,FUN-1,CD86,CD86,CD28LG2,

Tissue Specificity: Expressed by activated B-lymphocytes and monocytes.

Background: Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

Sequence Similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Molecular Weight: 60-80 kDa

Gene ID: 942

UniProt: P42081

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation