Growth Hormone 1 antibody (AA 27-216)

Catalog No. ABIN3042811

This Rabbit Polyclonal antibody specifically detects Growth Hormone 1 in IHC, WB and ELISA. It exhibits reactivity toward Rat.

Quick Overview for Growth Hormone 1 antibody (AA 27-216) (ABIN3042811)

Target: Growth Hormone 1 (GH1)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Growth Hormone 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Product Details anti-Growth Hormone 1 Antibody

Binding Specificity: AA 27-216

Purpose: Anti-Growth Hormone/GH1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Growth Hormone/GH1 Antibody (ABIN3042811). Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived rat GH recombinant protein (Position: F27-F216).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Niall, H. D., Hogan, M. L., Sauer, R., Rosenblum, I. Y., Greenwood, F. C. : Sequence of pituitary and placental lactogenic and growth hormones: evolution from a primordial peptide by gene reduplication. Proc. Nat. Acad. Sci. 68: 866-869, 1971. 2. Sundstrom, M., Lundqvist, T., Rodin, J., Giebel, L. B., Milligan, D., Norstedt, G. : Crystal structure of an antagonist mutant of human growth hormone, G120R, in complex with its receptor at 2.9 angstrom resolution. J. Biol. Chem. 271: 32197-32203, 1996.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Growth Hormone 1

Target: Growth Hormone 1 (GH1)

Alternative Name: GH1

Background:

Synonyms: Somatotropin,Growth hormone,Gh1,Gh,

Tissue Specificity: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. .

Background: Growth Hormone (GH) is mapped to 17q22-q24. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. Rat GH shares 98 % amino acid sequence homology with mouse. It binds two receptor molecules and thereby induces signal transduction through receptor dimerization. At high concentrations, GH acts as an antagonist because of a large difference in affinities at the respective binding sites.

Sequence Similarities: Belongs to the heparin-binding growth factors family.

Molecular Weight: 22 kDa

UniProt: P01244

Pathways: NF-kappaB Signaling, JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process