Interferon gamma antibody (AA 24-166)

Catalog No. ABIN3042815

The Rabbit Polyclonal anti-Interferon gamma antibody has been validated for WB and IHC. It is suitable to detect Interferon gamma in samples from Human. There are 6+ publications available.

Quick Overview for Interferon gamma antibody (AA 24-166) (ABIN3042815)

Target: Interferon gamma (IFNG)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Interferon gamma Antibody

Binding Specificity: AA 24-166

Purpose: Anti-IFN gamma Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-IFN gamma Antibody (ABIN3042815). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human IFN gamma recombinant protein (Position: Q24-Q166).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
ELISA , 0.1-0.5 μg/mL, Human, -
Western blot, 0.1-0.5 μg/mL, Human
1. Awad, M., Pravica, V., Perrey, C., El Gamel, A., Yonan, N., Sinnott, P. J., Hutchinson, I. V. CA repeat allele polymorphism in the first intron of the human interferon-gamma gene is associated with lung allograft fibrosis. Hum. Immunol. 60: 343-346, 1999. 2. Nathan, C. F., Murray, H. W., Wiebe, M. E., Rubin, B. Y. Identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity. J. Exp. Med. 158: 670-689, 1983. 3. Rossouw, M., Nel, H. J., Cooke, G. S., van Helden, P. D., Hoal, E. G. Association between tuberculosis and a polymorphic NF-kappa-B binding site in the interferon gamma gene. Lancet 361: 1871-1872, 2003. 4. Szabo, S. J., Sullivan, B. M., Stemmann, C., Satoskar, A. R., Sleckman, B. P., Glimcher, L. H. Distinct effects of T-bet in T(H)1 lineage commitment and IFN-gamma production in CD4 and CD8 T cells. Science 295: 338-342, 2002. 5. Zohlnhofer, D., Richter, T., Neumann, F.-J., Nuhrenberg, T., Wessely, R., Brandl, R., Murr, A., Klein, C. A., Baeuerle, P. A. Transcriptome analysis reveals a role of interferon-gamma in human neointima formation. Molec. Cell 7: 1059-1069, 2001.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide. Carrier free (No BSA)

Preservative: Sodium azide, Without preservative

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Interferon gamma antibody (ABIN3042815)

Chen, Zhao, Zhang, Yang, Zhao, Zhang, Yan, Pan, Li, Zhang, Li: "Tangshen Formula Attenuates Colonic Structure Remodeling in Type 2 Diabetic Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 4064156, (2017) (PubMed).

Li, Wu, Hang, Zhang, Li: "Women with recurrent spontaneous abortion have decreased 25(OH) vitamin D and VDR at the fetal-maternal interface." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, Vol. 50, Issue 11, pp. e6527, (2017) (PubMed).

Liu, Tan, Hu, Wu, Wang, Liu, Tang: "Somatostatin Improved B Cells Mature in Macaques during Intestinal Ischemia-Reperfusion." in: PLoS ONE, Vol. 10, Issue 7, pp. e0133692, (2016) (PubMed).

Ma, Chen, Bo, Lu, Zhang: "Protective effect of carnosine after chronic cerebral hypoperfusion possibly through suppressing astrocyte activation." in: American journal of translational research, Vol. 7, Issue 12, pp. 2706-15, (2016) (PubMed).

Yan, Wang, Liang, Fu, Guo: "HPV16L1-attenuated Shigella recombinant vaccine induced strong vaginal and systemic immune responses in guinea pig model." in: Human vaccines & immunotherapeutics, Vol. 10, Issue 12, pp. 3491-8, (2015) (PubMed).

Liu, Tan, Hu, Wu, Wang, Tang: "Somatostatin inhibits the production of interferon-γ by intestinal epithelial cells during intestinal ischemia-reperfusion in macaques." in: Digestive diseases and sciences, Vol. 59, Issue 10, pp. 2423-32, (2014) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Background:

Synonyms: Interferon gamma,IFN-gamma,Immune interferon,IFNG,

Tissue Specificity: Released primarily from activated T lymphocytes.

Background: Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Sequence Similarities: Belongs to the type II (or gamma) interferon family.

Molecular Weight: 17 kDa

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy