PFN2 antibody (C-Term)

Catalog No. ABIN3042861

Product Details anti-PFN2 Antibody

Target: PFN2 (Profilin 2 (PFN2))

Binding Specificity: AA 125-140, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: Anti-Profilin 2/PFN2 Antibody Picoband®

Sequence: NKKAYSMAKY LRDSGF

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Profilin 2/PFN2 Antibody (ABIN3042861). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Profilin 2, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Gieselmann, R., Kwiatkowski, D. J., Janmey, P. A., Witke, W. Distinct biochemical characteristics of the two human profilin isoforms. Europ. J. Biochem. 229: 621-628, 1995. 2. Joensuu, T., Blanco, G., Pakarinen, L., Sistonen, P., Kaariainen, H., Brown, S., de la Chapelle, A., Sankila, E.-M. Refined mapping of the Usher syndrome type III locus on chromosome 3, exclusion of candidate genes, and identification of the putative mouse homologous region. Genomics 38: 255-263, 1996.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PFN2

Target: PFN2 (Profilin 2 (PFN2))

Alternative Name: PFN2 (PFN2 Products)

Background:

Synonyms: Profilin-2,Profilin II,PFN2,

Tissue Specificity: Highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver.

Background: Profilin-2, also called PFN2, is a protein that in humans is encoded by the PFN2 gene. The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. This gene is mapped to 3q25.1. It is though to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. It binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.

Sequence Similarities: Contains 17 laminin EGF-like domains.

Molecular Weight: 15 kDa

UniProt: P35080

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis