E2F3 antibody (C-Term)

Catalog No. ABIN3042877

This Rabbit Polyclonal antibody specifically detects E2F3 in WB. It exhibits reactivity toward Human and Mouse and has been mentioned in 2+ publications.

Quick Overview for E2F3 antibody (C-Term) (ABIN3042877)

Target: E2F3

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-E2F3 Antibody

Binding Specificity: AA 446-465, C-Term

Purpose: Anti-Transcription factor E2F3 E2F3 Antibody Picoband®

Sequence: DLFDAYDLEK LPLVEDFMCS

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Transcription factor E2F3 E2F3 Antibody (ABIN3042877). Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human E2F3, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse
1. Chen, D., Pacal, M., Wenzel, P., Knoepfler, P. S., Leone, G., Bremner, R. Division and apoptosis of E2f-deficient retinal progenitors. Nature 462: 925-929, 2009. 2. Chong, J.-L., Wenzel, P. L., Saenz-Robles, M. T., Nair, V., Ferrey, A., Hagan, J. P., Gomez, Y. M., Sharma, N., Chen, H.-Z., Ouseph, M., Wang, S.-H., Trikha, P., and 10 others. E2f1-3 switch from activators in progenitor cells to repressors in differentiating cells. Nature 462: 930-934, 2009.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-E2F3 antibody (ABIN3042877)

Xie, Li, Wang, Jiao, Zhang, Zhang: "miRNA-regulated expression of oncogenes and tumor suppressor genes in the cisplatin-inhibited growth of K562 cells." in: Oncology reports, Vol. 23, Issue 6, pp. 1693-700, (2010) (PubMed).

Wang, Li, Zhang, Li, Yue, Xie, Xie: "Regulating A549 cells growth by ASO inhibiting miRNA expression." in: Molecular and cellular biochemistry, Vol. 339, Issue 1-2, pp. 163-71, (2010) (PubMed).

Target Details for E2F3

Target: E2F3

Background:

Synonyms: Transcription factor E2F3,E2F-3,E2F3,KIAA0075,

Background: Transcription factor E2F3, also known as KIAA0075, is a protein that in humans is encoded by the E2F3 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. By fluorescence in situ hybridization, E2F3 gene is mapped to 6q22.3. The induction of specific E2F activities is an essential component in the MYC pathways that control cell proliferation and cell fate decisions. This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts ly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle.

Sequence Similarities: Belongs to the E2F/DP family.

Molecular Weight: 49 kDa

UniProt: O00716