BAG2 antibody (N-Term)

Catalog No. ABIN3042900

Product Details anti-BAG2 Antibody

Target: BAG2 (BCL2-Associated Athanogene 2 (BAG2))

Binding Specificity: AA 1-14, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAG2 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for BAG family molecular chaperone regulator 2(BAG2) detection. Tested with WB in Human,Mouse,Rat.

Sequence: MAQAKINAKA NEGR

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for BAG family molecular chaperone regulator 2(BAG2) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: BCL2-associated athanogene 2
Protein Name: BAG family molecular chaperone regulator 2

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human BAG2(1-14aa MAQAKINAKANEGR), different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for BAG2 is approximately 0.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BAG2

Target: BAG2 (BCL2-Associated Athanogene 2 (BAG2))

Alternative Name: BAG2 (BAG2 Products)

Background: BAG family molecular chaperone regulator 2 is a protein that in humans is encoded by the BAG2 gene. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. The functional antagonisms displayed between BAG family proteins and Hip suggest that a proper balance of these 2 types of protein is required for achieving optimal cycles of substrate binding and release required for inducting conformational changes in proteins, with Hip promoting peptide substrate binding by Hsc70/Hsp70 and BAG family proteins promoting dissociation.

Synonyms: BAG 2 antibody|BAG family molecular chaperone regulator 2 antibody|BAG-2 antibody|BAG-family molecular chaperone regulator-2 antibody|Bag2 antibody|BAG2_HUMAN antibody|BCL 2 associated athanogene 2 antibody|Bcl-2-associated athanogene 2 antibody|BCL2 associated athanogene 2 antibody|BCL2-associated athanogene 2 antibody|dJ417I1.2(BAG family molecular chaperone regulator 2) antibody|dJ417I1.2 antibody|KIAA0576 antibody|MGC149462 antibody|OTTHUMP00000016668 antibody

UniProt: O95816