BAG2 antibody (C-Term)

Catalog No. ABIN2775278

This Rabbit Polyclonal antibody specifically detects BAG2 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Rabbit, Cow, Guinea Pig and Horse.It has been mentioned in 1 publication

Quick Overview for BAG2 antibody (C-Term) (ABIN2775278)

Target: BAG2 (BCL2-Associated Athanogene 2 (BAG2))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Rabbit, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-BAG2 Antibody

Binding Specificity: C-Term

Purpose: BAG2 Antibody - C-terminal region

Sequence: VDQKFQSIVI GCALEDQKKI KRRLETLLRN IENSDKAIKL LEHSKGAGSK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against BAG2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human BAG2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42177-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-BAG2 antibody (ABIN2775278)

Yue, Zhao, Liu, Zhang, Yu, Wang, Zheng, Liu, Li, Feng, Hu: "BAG2 promotes tumorigenesis through enhancing mutant p53 protein levels and function." in: eLife, Vol. 4, (2015) (PubMed).

Target Details for BAG2

Target: BAG2 (BCL2-Associated Athanogene 2 (BAG2))

Alternative Name: BAG2

Background:

Background Information: BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.

Gene Name: BCL2-associated athanogene 2

Alternative Symbols: BAG-2, dJ417I1.2

Protein Name: BAG family molecular chaperone regulator 2

Molecular Weight: 24 kDa

Gene ID: 9532

NCBI Accession: NP_004273

UniProt: O95816