ABI2 antibody (N-Term)

Catalog No. ABIN3042919

This Rabbit Polyclonal antibody specifically detects ABI2 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ABI2 antibody (N-Term) (ABIN3042919)

Target: ABI2 (Abl-Interactor 2 (ABI2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABI2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ABI2 Antibody

Binding Specificity: AA 158-173, N-Term

Purpose: Anti-Abl interactor 2 ABI2 Antibody Picoband®

Sequence: RFKVSTQNMK MGGLPR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Abl interactor 2 ABI2 Antibody (ABIN3042919). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ABI2, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Dai, Z., Pendergast, A. M. Abi-2, a novel SH3-containing protein interacts with the c-Abl tyrosine kinase and modulates c-Abl transforming activity. Genes Dev. 9: 2569-2582, 1995. 2. Machado, R. D., Pauciulo, M. W., Fretwell, N., Veal, C., Thomson, J. R., Guell, C. V., Aldred, M., Brannon, C. A., the International PPH Consortium, Trembath, R. C., Nichols, W. C. A physical and transcript map based upon refinement of the critical interval for PPH1, a gene for familial primary pulmonary hypertension. Genomics 68: 220-228, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ABI2

Target: ABI2 (Abl-Interactor 2 (ABI2))

Alternative Name: ABI2

Background:

Synonyms: Abl interactor 2,Abelson interactor 2,Abi-2,Abl-binding protein 3,AblBP3,Arg-binding protein 1,ArgBP1,ABI2,ARGBPIA,

Tissue Specificity: Highly expressed in brain tissues with the exception of the spinal cord. .

Background: ABI2 (ABL Interactor 2), is a protein that in humans is encoded by the ABI2 gene. By analysis of a YAC and a BAC, Machado et al. (2000) mapped the ABI2 gene to 2q31-q33. ABI2 possesses a basic N terminus with homology to a homeodomain protein, a central serine-rich region, 3 PEST sequences, which are implicated in susceptibility to protein degradation, several proline-rich stretches, and an acidic C terminus with multiple phosphorylation sites and an SH3 domain. Dai and Pendergast (1995) suggested that the ABI proteins may function to coordinate the cytoplasmic and nuclear functions of the ABL1 tyrosine kinase.

Sequence Similarities: Belongs to the ABI family.

Molecular Weight: 60 kDa