Flotillin 1 antibody (Middle Region)

Catalog No. ABIN3042941

The Rabbit Polyclonal anti-Flotillin 1 antibody has been validated for WB, IHC and IP. It is suitable to detect Flotillin 1 in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for Flotillin 1 antibody (Middle Region) (ABIN3042941)

Target: Flotillin 1 (FLOT1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Flotillin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-Flotillin 1 Antibody

Binding Specificity: AA 219-234, Middle Region

Purpose: Anti-Flotillin 1/FLOT1 Antibody Picoband®

Sequence: KKAAYDIEVN TRRAQA

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Flotillin 1/FLOT1 Antibody (ABIN3042941). Tested in IP, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Flotillin 1, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
1. Bickel, P. E., Scherer, P. E., Schnitzer, J. E., Oh, P., Lisanti, M. P., Lodish, H. F. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J. Biol. Chem. 272: 13793-13802, 1997. 2. Hazarika, P., Dham, N., Patel, P., Cho, M., Weidner, D., Goldsmith, L., Duvic, M. Flotillin 2 is distinct from epidermal surface antigen (ESA) and is associated with filopodia formation. J. Cell. Biochem. 75: 147-159, 1999. 3. Santamaria, A., Castellanos, E., Gomez, V., Benedit, P., Renau-Piqueras, J., Morote, J., Reventos, J., Thomson, T. M., Paciucci, R. PTOV1 enables the nuclear translocation and mitogenic activity of flotillin-1, a major protein of lipid rafts. Molec. Cell. Biol. 25: 1900-1911, 2005.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Flotillin 1 antibody (ABIN3042941)

Shi, Li, Liu, Yin, Zhao: "β-Cyclodextrin induces the differentiation of resident cardiac stem cells to cardiomyocytes through autophagy." in: Biochimica et biophysica acta. Molecular cell research, Vol. 1864, Issue 8, pp. 1425-1434, (2017) (PubMed).

Target Details for Flotillin 1

Target: Flotillin 1 (FLOT1)

Alternative Name: FLOT1

Background:

Synonyms: Flotillin-1,FLOT1,

Tissue Specificity: Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). .

Background: FLOT1 (Flotillin 1), is a protein that in humans is encoded by the FLOT1 gene. The International Radiation Hybrid Mapping Consortium mapped the FLOT1 gene to chromosome 6. Bickel et al. (1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae. It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes. Hazarika et al. (1999) found that stable transfection of Flot1, which they called ESA/flotillin-2, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells. Santamaria et al. (2005) found that prostate tumor overexpressed gene-1 interacted with flotillin-1 in detergent-insoluble membrane fractions. Flotillin-1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase.

Sequence Similarities: Belongs to the eIF-3 subunit B family.

Molecular Weight: 47 kDa

UniProt: O75955