EIF3B antibody (C-Term)

Catalog No. ABIN3042942

This anti-EIF3B antibody is a Rabbit Polyclonal antibody detecting EIF3B in WB. Suitable for Human.

Quick Overview for EIF3B antibody (C-Term) (ABIN3042942)

Target: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF3B Antibody

Binding Specificity: AA 755-775, C-Term

Purpose: Anti-eIF3B Antibody Picoband®

Sequence: EDFRKYRKMA QELYMEQKNE R

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-eIF3B Antibody (ABIN3042942). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human eIF3B, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Asano, K., Kinzy, T. G., Merrick, W. C., Hershey, J. W. B. Conservation and diversity of eukaryotic translation initiation factor eIF3. J. Biol. Chem. 272: 1101-1109, 1997. 2. Chaudhuri, J., Chakrabarti, A., Maitra, U. Biochemical characterization of mammalian translation initiation factor 3 (eIF3): molecular cloning reveals that p110 subunit is the mammalian homologue of saccharomyces cerevisiae protein Prt1. J. Biol. Chem. 272: 30975-30983, 1997. 3. Methot, N., Rom, E., Olsen, H., Sonenberg, N. The human homologue of the yeast Prt1 protein is an integral part of the eukaryotic initiation factor 3 complex and interacts with p170. J. Biol. Chem. 272: 1110-1116, 1997.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EIF3B

Target: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B))

Alternative Name: EIF3B

Background:

Synonyms: Eukaryotic translation initiation factor 3 subunit B ,eIF3b ,Eukaryotic translation initiation factor 3 subunit 9 ,Prt1 homolog,hPrt1,eIF-3-eta ,eIF3 p110 ,eIF3 p116,EIF3B ,EIF3S9 ,

Background: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B), also called PRT1 or EIF3-p116, is a protein that in humans is encoded by the EIF3B gene. By searching an EST database, Methot et al. (1997) identified a cDNA encoding a human Prt1 homolog. Asano et al. (1997) demonstrated that the 115-kD component of HeLa cell eIF3 is actually composed of 2 proteins, p116 (PRT1) and an unrelated 110-kD protein. Chaudhuri et al. (1997) isolated cDNAs encoding PRT1, which they called p110 based on the size of the corresponding component of rabbit eIF3.

Sequence Similarities: Belongs to the eIF-3 subunit B family.

Molecular Weight: 115 kDa

UniProt: P55884

Pathways: Ribonucleoprotein Complex Subunit Organization