CXCR5 antibody (C-Term)

Catalog No. ABIN3042947

The Rabbit Polyclonal anti-CXCR5 antibody has been validated for WB. It is suitable to detect CXCR5 in samples from Human, Mouse and Rat.

Quick Overview for CXCR5 antibody (C-Term) (ABIN3042947)

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CXCR5 Antibody

Binding Specificity: AA 358-372, C-Term

Purpose: Anti-CXCR5 Antibody Picoband®

Sequence: SSLSESENAT SLTTF

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CXCR5 Antibody (ABIN3042947). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CXCR5, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Allen, C. D. C., Ansel, K. M., Low, C., Lesley, R., Tamamura, H., Fujii, N., Cyster, J. G. Germinal center dark and light zone organization is mediated by CXCR4 and CXCR5. Nature Immun. 5: 943-952, 2004. 2. Chan, C.-C., Shen, D., Hackett, J. J., Buggage, R. R., Tuaillon, N. Expression of chemokine receptors, CXCR4 and CXCR5, and chemokines, BLC and SDF-1, in the eyes of patients with primary intraocular lymphoma. Ophthalmology 110: 421-426, 2003. 3. Reif, K., Ekland, E. H., Ohl, L., Nakano, H., Lipp, M., Forster, R., Cyster, J. G. Balanced responsiveness to chemoattractants from adjacent zones determines B-cell position. Nature 416: 94-99, 2002.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CXCR5

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Alternative Name: CXCR5

Background:

Synonyms: C-X-C chemokine receptor type 5,CXC-R5,CXCR-5,Burkitt lymphoma receptor 1,Monocyte-derived receptor 15,MDR-15,CD185,CXCR5,BLR1, MDR15,

Tissue Specificity: Expression in mature B-cells and Burkitt lymphoma cells.

Background: CXCR5 (Chemokine CXC Motif Receptor 5), also known as BLR1, is a G protein-coupled seven transmembrane receptor for chemokine CXCL13 (also known as BLC) and belongs to the CXC chemokine receptor family. In humans, the CXC-R5 protein is encoded by the CXCR5 gene. The gene plays an essential role in B cell migration. Reif et al. (2002) concluded that their findings defined the mechanism of B-cell relocalization in response to antigen, and established that cell position in vivo can be determined by the balance of responsiveness to chemoattractants made in separate but adjacent zones. Chan et al. (2003) investigated the expression of chemokines and chemokine receptors in eyes with primary intraocular B-cell lymphoma (PIOL).

Sequence Similarities: Belongs to the G-protein coupled receptor 1 family.

Molecular Weight: 22 kDa

UniProt: P32302