SCLY antibody (C-Term)

Catalog No. ABIN3042960

This anti-SCLY antibody is a Rabbit Polyclonal antibody detecting SCLY in WB. Suitable for Human.

Quick Overview for SCLY antibody (C-Term) (ABIN3042960)

Target: SCLY (Selenocysteine Lyase (SCLY))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCLY antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SCLY Antibody

Binding Specificity: AA 432-445, C-Term

Purpose: Anti-Selenocysteine lyase SCLY Antibody Picoband®

Sequence: QDLKQAVAQL EDQA

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Selenocysteine lyase SCLY Antibody (ABIN3042960). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SCLY.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Mihara, H., Kurihara, T., Watanabe, T., Yoshimura, T., Esaki, N. cDNA cloning, purification, and characterization of mouse liver selenocysteine lyase: candidate for selenium delivery protein in selenoprotein synthesis. J. Biol. Chem. 275: 6195-6200, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SCLY

Target: SCLY (Selenocysteine Lyase (SCLY))

Alternative Name: SCLY

Background:

Synonyms: Selenocysteine lyase,hSCL,4.4.1.16,SCLY,SCL,

Tissue Specificity: Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung. .

Background: SCLY (Selenocysteine Lyase), is an enzyme that catalyzes the chemical reaction. This enzyme belongs to the family of lyases, specifically the class of carbon-sulfur lyases. The International Radiation Hybrid Mapping Consortium mapped the human SCLY gene to chromosome 2. Mihara et al. (2000) showed that mouse Scly catalyzed the conversion of L-selenocysteine to L-alanine. Scly activity required pyridoxal 5-prime phosphate, was specific to L-selenocysteine, and showed maximum reactivity at pH 9.0.

Sequence Similarities: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Molecular Weight: 85-91 kDa

UniProt: Q96I15

Pathways: Stem Cell Maintenance