Cullin 3 antibody (Middle Region)

Catalog No. ABIN3042989

The Rabbit Polyclonal anti-Cullin 3 antibody has been validated for WB. It is suitable to detect Cullin 3 in samples from Human, Mouse and Rat.

Quick Overview for Cullin 3 antibody (Middle Region) (ABIN3042989)

Target: Cullin 3 (CUL3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Cullin 3 Antibody

Binding Specificity: AA 426-444, Middle Region

Purpose: Anti-Cullin 3/CUL3 Antibody Picoband®

Sequence: DVFERYYKQH LARRLLTNK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cullin 3/CUL3 Antibody (ABIN3042989). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Cullin 3, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Kigoshi, Y., Tsuruta, F., Chiba, T. Ubiquitin ligase activity of Cul3-KLHL7 protein is attenuated by autosomal dominant retinitis pigmentosa causative mutation. J. Biol. Chem. 286: 33613-33621, 2011. 2. Kipreos, E. T., Lander, L. E., Wing, J. P., He, W. W., Hedgecock, E. M. cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85: 829-839, 1996. 3. Rondou, P., Haegeman, G., Vanhoenacker, P., Van Craenenbroeck, K. BTB protein KLHL12 targets the dopamine D4 receptor for ubiquitination by a Cul3-based E3 ligase. J. Biol. Chem. 283: 11083-11096, 2008. 4. Sumara, I., Quadroni, M., Frei, C., Olma, M. H., Sumara, G., Ricci, R., Peter, M. A Cul3-based E3 ligase removes Aurora B from mitotic chromosomes, regulating mitotic progression and completion of cytokinesis in human cells. Dev. Cell 12: 887-900, 2007.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cullin 3

Target: Cullin 3 (CUL3)

Alternative Name: CUL3

Background:

Synonyms: Cullin-3,CUL-3,CUL3,KIAA0617,

Tissue Specificity: Widely expressed.

Background: Cullin-3 (Cul 3), is a protein that in humans is encoded by the CUL3 gene. Kipreos et al. (1996) identified a conserved gene family, designated cullins, with at least 5 members in nematodes, 6 in humans, and 3 in S. cerevisiae. Human CUL3 is an ortholog of nematode cul3. Hartz (2012) mapped the CUL3 gene to chromosome 2q36.2 based on an alignment of the CUL3 sequence with the genomic sequence (GRCh37). Sumara et al. (2007) found that KLHL9, KLHL13, and CUL3 interacted ly in a 370-kD protein complex in HeLa cell lysates. Rondou et al. (2008) showed that interaction between KLHL12 and the CUL3 ubiquitin ligase complex ed ubiquitination of dopamine receptor D4 (DRD4). KLHL12 interacted ly with CUL3 and with the polymorphic intracellular loop-3 of D4. By immunoprecipitation analysis of HeLa cells and HCT116 human colon carcinoma cells expressing epitope-tagged proteins, Kigoshi et al. (2011) showed that KLHL7 interacted with CUL3 and ROC1 (RBX1).

Sequence Similarities: Belongs to the cullin family.

Molecular Weight: 89 kDa

UniProt: Q13618

Pathways: M Phase