HSC70 Interacting Protein HIP antibody (N-Term)

Catalog No. ABIN3042991

This Rabbit Polyclonal antibody specifically detects HSC70 Interacting Protein HIP in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for HSC70 Interacting Protein HIP antibody (N-Term) (ABIN3042991)

Target: HSC70 Interacting Protein HIP (ST13) (Suppression of Tumorigenicity 13 (ST13))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSC70 Interacting Protein HIP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-HSC70 Interacting Protein HIP Antibody

Binding Specificity: AA 1-19, N-Term

Purpose: Anti-HSC70 Interacting Protein HIP/ST13 Antibody Picoband®

Sequence: MDPRKVNELR AFVKMCKQD

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HSC70 Interacting Protein HIP/ST13 Antibody (ABIN3042991). Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ST13, different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Hohfeld, J., Minami, Y., Hartl, F.-U. Hip, a novel cochaperone involved in the eukaryotic Hsc70/Hsp40 reaction cycle. Cell 83: 589-598, 1995. 2. Prapapanich, V., Chen, S., Nair, S. C., Rimerman, R. A., Smith, D. F. Molecular cloning of human p48, a transient component of progesterone receptor complexes and an Hsp70-binding protein. Molec. Endocr. 10: 420-431, 1996. 3. Zhang, Y., Cai, X., Schlegelberger, B., Zheng, S. Assignment of human putative tumor suppressor genes ST13 (alias SNC6) and ST14 (alias SNC19) to human chromosome bands 22q13 and 11q24-q25 by in situ hybridization. Cytogenet. Cell Genet. 83: 56-57, 1998.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HSC70 Interacting Protein HIP

Target: HSC70 Interacting Protein HIP (ST13) (Suppression of Tumorigenicity 13 (ST13))

Alternative Name: ST13

Background:

Synonyms: Hsc70-interacting protein,Hip,Aging-associated protein 2,Progesterone receptor-associated p48 protein,Protein FAM10A1,Putative tumor suppressor ST13,Renal carcinoma antigen NY-REN-33,Suppression of tumorigenicity 13 protein,ST13,AAG2, FAM10A1, HIP, SNC6,

Tissue Specificity: Widely expressed in different tissues.

Background: ST13 (Suppression of Tumorigenicity 13), also known as P48 or HIP, is a protein that in humans is encoded by the ST13 gene. ST13 is an abundant, highly conserved protein that binds the major cytosolic chaperones heat-shock protein 70-kD (HSP70) and HSP90 during an intermediate stage of steroid receptor assembly, but is absent from the mature receptor complex. Zhang et al. (1998) mapped the ST13 gene to chromosome 22q13 by fluorescence in situ hybridization. They noted that colorectal, breast, and ovarian carcinomas frequently show loss of heterozygosity at this site. Using a yeast 2-hybrid assay, Hohfeld et al. (1995) showed that rat Hip bound Hsc70 (HSPA8). One Hip oligomer bound the ATPase domains of at least 2 Hsc70 Molecules, and binding was dependent on activation of the Hsc70 ATPase by Hsp40 (DNAJB1). Hip stabilized the ADP-bound form of Hsc70, which had a high affinity for a test protein substrate. Hohfeld et al. (1995) concluded that HIP contributes to interactions of HSC70 with target proteins through its own chaperone activity.

Sequence Similarities: Belongs to the FAM10 family.

Molecular Weight: 45-54 kDa

UniProt: P50502