NOXA1 antibody (Middle Region)

Catalog No. ABIN3042993

Product Details anti-NOXA1 Antibody

Target: NOXA1 (NADPH Oxidase Activator 1 (NOXA1))

Binding Specificity: AA 176-195, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOXA1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for NADPH oxidase activator 1(NOXA1) detection. Tested with WB in Human.

Sequence: RQVPRGEVFR PHRWHLKHLE

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for NADPH oxidase activator 1(NOXA1) detection. Tested with WB in Human.
Gene Name: NADPH oxidase activator 1
Protein Name: NADPH oxidase activator 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NOXA1(176-195aa RQVPRGEVFRPHRWHLKHLE).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NOXA1

Target: NOXA1 (NADPH Oxidase Activator 1 (NOXA1))

Alternative Name: NOXA1 (NOXA1 Products)

Background: NOXA1(NADPH oxidase activator 1), also called NOX ACTIVATOR 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz(2007) mapped the NOXA1 gene to chromosome 9q34.3 based on an alignment of the NOXA1 sequence with the genomic sequence(build 36.1). Banfi et al.(2003) mapped the mouse Noxa1 gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al.(2003) showed that human p51-NOX interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that p51-NOX bound GTP-bound RAC1, but not GDP-bound RAC1. p51-NOX also bound p47-PHOX(NCF1) and p41-NOX(NOXO1), and trp436 within the SH3 domain of p51-NOX was required for these interactions. Human cell lines or COS-7 cells cotransfected with p51-NOX and p41-NOX and either gp91-PHOX(CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.

Synonyms: Antigen NY CO 31 antibody|Antigen NY-CO-31 antibody|FLJ25475 antibody|Inhibitory NADPH oxidase activator 1 antibody|MGC131800 antibody|NADPH oxidase activator 1 antibody|NCF2 like protein antibody|NCF2-like protein antibody|NOX activator 1 antibody|NOXA 1 antibody|Noxa1 antibody|NOXA1_HUMAN antibody|NY CO 31 antibody|p51 nox antibody|p51-nox antibody|p51NOX antibody|P67phox like factor antibody|P67phox-like factor antibody|SDCCAG31 antibody|Serologically defined colon cancer antigen 31 antibody