NOXA1 antibody (Middle Region)

Catalog No. ABIN3042993

This anti-NOXA1 antibody is a Rabbit Polyclonal antibody detecting NOXA1 in WB. Suitable for Human.

Quick Overview for NOXA1 antibody (Middle Region) (ABIN3042993)

Target: NOXA1 (NADPH Oxidase Activator 1 (NOXA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOXA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NOXA1 Antibody

Binding Specificity: AA 176-195, Middle Region

Purpose: Anti-NOXA1 Antibody Picoband®

Sequence: RQVPRGEVFR PHRWHLKHLE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NOXA1 Antibody (ABIN3042993). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NOXA1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Banfi, B., Clark, R. A., Steger, K., Krause, K.-H. Two novel proteins activate superoxide generation by the NADPH oxidase NOX1. J. Biol. Chem. 278: 3510-3513, 2003. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 7/25/2007. 3. Takeya, R., Ueno, N., Kami, K., Taura, M., Kohjima, M., Izaki, T., Nunoi, H., Sumimoto, H. Novel human homologues of p47(phox) and p67(phox) participate in activation of superoxide-producing NADPH oxidases. J. Biol. Chem. 278: 25234-25246, 2003.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NOXA1

Target: NOXA1 (NADPH Oxidase Activator 1 (NOXA1))

Alternative Name: NOXA1

Background:

Synonyms: NADPH oxidase activator 1,NOX activator 1,Antigen NY-CO-31,NCF2-like protein,P67phox-like factor,p51-nox,NOXA1,P51NOX,

Tissue Specificity: Widely expressed. Detected in pancreas, liver, kidney, spleen, prostate, small intestine and colon. .

Background: NOXA1 (NADPH oxidase activator 1), also called NOX ACTIVATOR 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz (2007) mapped the NOXA1 gene to chromosome 9q34.3 based on an alignment of the NOXA1 sequence with the genomic sequence (build 36.1). Banfi et al. (2003) mapped the mouse Noxa1 gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al. (2003) showed that human p51-NOX interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that p51-NOX bound GTP-bound RAC1, but not GDP-bound RAC1. p51-NOX also bound p47-PHOX (NCF1) and p41-NOX (NOXO1), and trp436 within the SH3 domain of p51-NOX was required for these interactions. Human cell lines or COS-7 cells cotransfected with p51-NOX and p41-NOX and either gp91-PHOX (CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.

Sequence Similarities: Belongs to the NCF2/NOXA1 family.

Molecular Weight: 60 kDa