IDS antibody (C-Term)

Catalog No. ABIN3042998

The Rabbit Polyclonal anti-IDS antibody has been validated for WB and IHC. It is suitable to detect IDS in samples from Human.

Quick Overview for IDS antibody (C-Term) (ABIN3042998)

Target: IDS (Iduronate 2-Sulfatase (IDS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-IDS Antibody

Binding Specificity: AA 430-448, C-Term

Purpose: Anti-Iduronate 2 sulfatase/IDS Antibody Picoband®

Sequence: ELCREGKNLL KHFRFRDLE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Iduronate 2 sulfatase/IDS Antibody (ABIN3042998). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Iduronate 2 sulfatase.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Daniele, A., Faust, C. J., Herman, G. E., Di Natale, P., Ballabio, A. Cloning and characterization of the cDNA for the murine iduronate sulfatase gene. Genomics 16: 755-757, 1993. 2. Faust, C. J., Verkerk, A. J. M. H., Wilson, P. J., Morris, C. P., Hopwood, J. J., Oostra, B. A., Herman, G. E. Genetic mapping on the mouse X chromosome of human cDNA clones for the fragile X and Hunter syndromes. Genomics 12: 814-817, 1992. 3. Wilson, P. J., Suthers, G. K., Callen, D. F., Baker, E., Nelson, P. V., Cooper, A., Wraith, J. E., Sutherland, G. R., Morris, C. P., Hopwood, J. J. Frequent deletions at Xq28 indicate genetic heterogeneity in Hunter syndrome. Hum. Genet. 86: 505-508, 1991.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for IDS

Target: IDS (Iduronate 2-Sulfatase (IDS))

Alternative Name: IDS

Background:

Synonyms: Iduronate 2-sulfatase,3.1.6.13,Alpha-L-iduronate sulfate sulfatase,Idursulfase,Iduronate 2-sulfatase 42 kDa chain,Iduronate 2-sulfatase 14 kDa chain,IDS,SIDS,

Tissue Specificity: Liver, kidney, lung, and placenta.

Background: IDS (Iduronate-2-sulfatase) is a sulfatase enzyme associated with Hunter syndrome. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. Wilson et al. (1991) used an IDS cDNA clone to localize the gene to Xq28, distal to the fragile X site. Faust et al. (1992) and Daniele et al. (1993) demonstrated that the homologous Ids gene in the mouse occupies the same position on the X chromosome in relation to the FMR1, F9, and GABRA3 genes. Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked mucopolysaccharidosis type II, also known as Hunter syndrome. Iduronate-2-sulfatase has a strong sequence homology with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase.

Sequence Similarities: Contains 2 follistatin-like domains.

Molecular Weight: 22-24 kDa

UniProt: P22304

Pathways: Glycosaminoglycan Metabolic Process