CD1a antibody (Middle Region)

Catalog No. ABIN3043011

This anti-CD1a antibody is a Rabbit Polyclonal antibody detecting CD1a in WB. Suitable for Human.

Quick Overview for CD1a antibody (Middle Region) (ABIN3043011)

Target: CD1a

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD1a antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD1a Antibody

Binding Specificity: AA 158-173, Middle Region

Purpose: Anti-CD1a Antibody Picoband®

Sequence: AKHFCKVLNQ NQHEND

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CD1a Antibody (ABIN3043011). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human CD1a.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Martin, L. H., Calabi, F., Lefebvre, F.-A., Bilsland, C. A. G., Milstein, C. Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c.Proc. Nat. Acad. Sci. 84: 9189-9193, 1987. 2. Moody, D. B., Young, D. C., Cheng, T.-Y., Rosat, J.-P., Roura-mir, C., O'Connor, P. B., Zajonc, D. M., Walz, A., Miller, M. J., Levery, S. B., Wilson, I. A., Costello, C. E., Brenner, M. B. T cell activation by lipopeptide antigens. Science 303: 527-531, 2004. Note: Erratum: Science 304: 211 only, 2004. 3. Park, S.-H., Bendelac, A. CD1-restricted T-cell responses and microbial infection. Nature 406: 788-792, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CD1a

Target: CD1a

Alternative Name: CD1A

Background:

Synonyms: T-cell surface glycoprotein CD1a;T-cell surface antigen T6/Leu-6;hTa1 thymocyte antigen;CD1a;CD1A;

Tissue Specificity: Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues. .

Background: CD1A (Thymocyte antigen CD1A) also known as CD1, is a human protein encoded by the CD1A gene. CD1A is 1 of 5 distinct CD1 genes that are variably conserved in different mammalian species. This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin.The CD1A gene is mapped on 1q23.1. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. CD1A is concentrated in the early or recycling endosome. Moody et al. (2004) reported that CD1A presents to T cells a family of previously unknown lipopeptides from Mycobacterium tuberculosis, named didehydroxymycobactins because of their structural relation to mycobactin siderophores. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Apolipoprotein E (107741) binds lipid antigens and delivers them by receptor-mediated uptake into endosomal compartments containing CD1 in antigen-presenting cells.

Sequence Similarities: Contains 9 SRCR domains.

Molecular Weight: 37 kDa

UniProt: P06126

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process