CD163 antibody (C-Term)

Catalog No. ABIN3043012

Product Details anti-CD163 Antibody

Target: CD163

Binding Specificity: AA 1092-1109, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD163 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Anti-CD163 Antibody Picoband®

Sequence: HQIQYREMNS CLNADDLD

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti- Antibody (ABIN3043012). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CD163.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Fabriek, B. O., van Bruggen, R., Deng, D. M., Ligtenberg, A. J. M., Nazmi, K., Schornagel, K., Vloet, R. P. M., Dijkstra, C. D., van den Berg, T. K. The macrophage scavenger receptor CD163 functions as an innate immune sensor for bacteria. Blood 113: 887-892, 2009. 2. Ritter, M., Buechler, C., Langmann, T., Schmitz, G.Genomic organization and chromosomal localization of the human CD163 (M130) gene: a member of the scavenger receptor cysteine-rich superfamily. Biochem. Biophys. Res. Commun. 260: 466-474, 1999. 3. Stover, C. M., Schleypen, J., Gronlund, J., Speicher, M. R., Schwaeble, W. J., Holmskov, U. Assignment of CD163B, the gene encoding M160, a novel scavenger receptor, to human chromosome 12p13.3 by in situ hybridization and somatic cell hybrid analysis. Cytogenet. Cell Genet. 90: 246-247, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CD163 antibody (ABIN3043012)

Liu, Xiao, Liu, Li, Zhou, Xian, Wang, Zhang, Wang, Ho, Zhuang: "SIV Infection Impairs the Central Nervous System in Chinese Rhesus Macaques." in: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, Vol. 11, Issue 3, pp. 592-600, (2017) (PubMed).

Huang, Chen, Wang, Wang, Ning, He, Hu, Yuan, Li, Wang, Liu, Chen, Ren, Sun: "Detecting cell-in-cell structures in human tumor samples by E-cadherin/CD68/CD45 triple staining." in: Oncotarget, Vol. 6, Issue 24, pp. 20278-87, (2016) (PubMed).

Target Details for CD163

Target: CD163

Alternative Name: CD163 (CD163 Products)

Background:

Synonyms: Scavenger receptor cysteine-rich type 1 protein M130,Hemoglobin scavenger receptor,CD163,Soluble CD163,sCD163,CD163,M130,

Tissue Specificity: Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood. .

Background: CD163 (Cluster of Differentiation 163) is a protein that in humans is encoded by the CD163 gene. The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Sequence Similarities: Contains 9 SRCR domains.

Molecular Weight: 150 kDa