Patched 2 antibody (C-Term)

Catalog No. ABIN3043014

This anti-Patched 2 antibody is a Rabbit Polyclonal antibody detecting Patched 2 in WB. Suitable for Human and Mouse.

Quick Overview for Patched 2 antibody (C-Term) (ABIN3043014)

Target: Patched 2 (PTCH2)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Patched 2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Patched 2 Antibody

Binding Specificity: AA 885-901, C-Term

Purpose: Anti-PTCH2 Antibody Picoband®

Sequence: WLHDKYDTTG ENLRIPP

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PTCH2 Antibody (ABIN3043014). Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PTCH2, different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse
1. Nieuwenhuis, E., Motoyama, J., Barnfield, P. C., Yoshikawa, Y., Zhang, X., Mo, R., Crackower, M. A., Hui, C. Mice with a targeted mutation of Patched 2 are viable but develop alopecia and epidermal hyperplasia. Molec. Cell. Biol. 26: 6609-6622, 2006.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Patched 2

Target: Patched 2 (PTCH2)

Alternative Name: PTCH2

Background:

Synonyms: Protein patched homolog 2;PTC2;PTCH2;UNQ560/PRO1121/PRO57079;

Tissue Specificity: Bone marrow, testis and kidney. Isoform 4 is detected in all bladder tumor and prostate tumor cells. .

Background: PTCH2 (patched 2), also known as PTC2, encodes a 1.203-amino acid putative transmembrane protein that is highly homologous to the PTCH product. The Patched-hedgehog signaling pathway is involved in establishing segment polarity and the anterior-posterior orientation of a number of developing appendages. The pathway is conserved from fly to vertebrates and has been implicated in a number of human neoplasms and developmental syndromes. Mutations in the human Patched gene are the basis of the nevoid basal cell carcinoma syndrome. With age, homozygous mutant male mice developed skin lesions consisting of alopecia and epidermal hyperplasia, suggesting a role for Ptch2 in adult epidermal homeostasis via Shh signaling.

Sequence Similarities: Belongs to the patched family.

Molecular Weight: 50 kDa

UniProt: Q9Y6C5

Pathways: Hedgehog Signaling