GSTP1 antibody (C-Term)

Catalog No. ABIN3043019

The Rabbit Polyclonal anti-GSTP1 antibody has been validated for WB and IHC. It is suitable to detect GSTP1 in samples from Human, Mouse and Rat. There are 3+ publications available.

Quick Overview for GSTP1 antibody (C-Term) (ABIN3043019)

Target: GSTP1 (Glutathione S-Transferase pi 1 (GSTP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSTP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GSTP1 Antibody

Binding Specificity: AA 197-210, C-Term

Purpose: Anti-GST3/GST pi/GSTP1 Antibody Picoband®

Sequence: PEYVNLPING NGKQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-GST3/GST pi/GSTP1 Antibody (ABIN3043019). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GST3, different from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
1. Morrow, C. S., Cowan, K. H., Goldsmith, M. E. : Structure of the human genomic glutathione S-transferase-pi gene. Gene 75: 3-11, 1989 2. Islam, M. Q., Platz, A., Szpirer, J., Szpirer, C., Levan, G., Mannervik, B. : Chromosomal localization of human glutathione transferase genes of classes alpha, mu and pi. Hum. Genet. 82: 338-342, 1989. 3. Konohana, A., Konohana, I., Schroeder, W. T., O'Brien, W. R., Amagai, M., Greer, J., Shimizu, N., Gammon, W. R., Siciliano, M. J., Duvic, M. :Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. J. Invest. Derm. 95: 119-126, 1990.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-GSTP1 antibody (ABIN3043019)

Zhang, Yan, Xu, Fang, Zhang, Zhang, Wu, Kong, Huang: "The recovery trajectory of adolescent social defeat stress-induced behavioral, (1)H-MRS metabolites and myelin changes in Balb/c mice." in: Scientific reports, Vol. 6, pp. 27906, (2018) (PubMed).

Sun, Hu, Xiong, Tu, Zhao, Chen, Song, Ye: "Enhanced expression of stem cell markers and drug resistance in sphere-forming non-small cell lung cancer cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 6, pp. 6287-300, (2016) (PubMed).

Yang, Gao, Zhang, Fang, Wu, Xu, Huang: "Changes in proinflammatory cytokines and white matter in chronically stressed rats." in: Neuropsychiatric disease and treatment, Vol. 11, pp. 597-607, (2015) (PubMed).

Target Details for GSTP1

Target: GSTP1 (Glutathione S-Transferase pi 1 (GSTP1))

Alternative Name: GSTP1

Background:

Synonyms: Glutathione S-transferase P,2.5.1.18,GST class-pi,GSTP1-1,GSTP1,FAEES3, GST3,

Tissue Specificity: Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. .

Background: Glutathione S-transferases pi, also known as GST3, present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. The GST-pi gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. The GST-pi gene is mapped to chromosome 11 .Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. GSTP does not contribute in vivo to the formation of glutathione conjugates of acetaminophen but plays a novel and unexpected role in the toxicity of this compound.

Sequence Similarities: Belongs to the GST superfamily. Pi family.

Molecular Weight: 23 kDa

UniProt: P09211

Pathways: Cellular Response to Molecule of Bacterial Origin