SSX2 antibody (C-Term)

Catalog No. ABIN3043034

The Rabbit Polyclonal anti-SSX2 antibody has been validated for WB and IHC. It is suitable to detect SSX2 in samples from Human.

Quick Overview for SSX2 antibody (C-Term) (ABIN3043034)

Target: SSX2 (Synovial Sarcoma, X Breakpoint 2 (SSX2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SSX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SSX2 Antibody

Binding Specificity: AA 157-184, C-Term

Purpose: Anti-Protein SSX2 SSX2 Antibody Picoband®

Sequence: NREAQEKEER RGTAHRWSSQ NTHNIGRF

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Protein SSX2 SSX2 Antibody (ABIN3043034). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Protein SSX2.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Crew, A. J., Clark, J., Fisher, C., Gill, S., Grimer, R., Chand, A., Shipley, J., Gusterson, B. A., Cooper, C. S. : Fusion of SYT to two genes, SSX1 and SSX2, encoding proteins with homology to the Kruppel-associated box in human synovial sarcoma. EMBO J. 14: 2333-2340, 1995.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SSX2

Target: SSX2 (Synovial Sarcoma, X Breakpoint 2 (SSX2))

Alternative Name: Protein SSX2

Background:

Synonyms: Protein SSX2,Cancer/testis antigen 5.2,CT5.2,Synovial sarcoma, X breakpoint 2,Tumor antigen HOM-MEL-40,SSX2,SSX2A,SSX2B,

Tissue Specificity: Expressed at high level in the testis. Expressed at low level in thyroid. Not detected in tonsil, colon, lung, spleen, prostate, kidney, striated and smooth muscles. Detected in rhabdomyosarcoma and fibrosarcoma cell lines. Not detected in mesenchymal and epithelial cell lines.

Background: Synovial sarcoma, X breakpoint 2, also known as SSX2, is a human gene. The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. SSX1, SSX2 and SSX4 genes have been involved in the t (X,18) translocation characteristically found in all synovial sarcomas. The SSX1 and SSX2 genes encode closely related proteins (81 % identity) of 188 amino acids that are rich in charged amino acids. The N-terminal portion of each SSX protein exhibits homology to the Kruppel-associated box (KRAB), a transcriptional repressor domain previously found only in Kruppel-type zinc finger proteins.

Sequence Similarities: Belongs to the SSX family.

Molecular Weight: 104 kDa

UniProt: Q16385