Growth Hormone Receptor antibody (Middle Region)

Catalog No. ABIN3043066

This Rabbit Polyclonal antibody specifically detects Growth Hormone Receptor in WB. It exhibits reactivity toward Mouse and Rat.

Quick Overview for Growth Hormone Receptor antibody (Middle Region) (ABIN3043066)

Target: Growth Hormone Receptor (GHR)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Growth Hormone Receptor antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Growth Hormone Receptor Antibody

Binding Specificity: AA 231-247, Middle Region

Purpose: Anti-Growth hormone receptor/GHR Antibody Picoband®

Sequence: DKEHEVRVRS RQRSFEK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Growth hormone receptor/GHR Antibody (ABIN3043066). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse Growth hormone receptor, identical to the related rat sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
1. Barton, D. E., Foellmer, B. E., Wood, W. I., Francke, U. : Chromosome mapping of the growth hormone receptor gene in man and mouse. Cytogenet. Cell Genet. 50: 137-141, 1989. 2. Godowski, P. J., Leung, D. W., Meacham, L. R., Galgani, J. P., Hellmiss, R., Keret, R., Rotwein, P. S., Parks, J. S., Laron, Z., Wood, W. I. : Characterization of the human growth hormone receptor gene and demonstration of a partial gene deletion in two patients with Laron-type dwarfism. Proc. Nat. Acad. Sci. 86: 8083-8087, 1989. 3. Amselem, S., Sobrier, M.-L., Duquesnoy, P., Rappaport, R., Postel-Vinay, M.-C., Gourmelen, M., Dallapiccola, B., Goossens, M. : Recurrent nonsense mutations in the growth hormone receptor from patients with Laron dwarfism. J. Clin. Invest. 87: 1098-1102, 1991.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Growth Hormone Receptor

Target: Growth Hormone Receptor (GHR)

Alternative Name: GHR

Background:

Synonyms: Growth hormone receptor,GH receptor,Somatotropin receptor,Growth hormone-binding protein,GH-binding protein,GHBP,Serum-binding protein,Ghr,

Tissue Specificity: Expressed in all tissues tested including, liver, heart, adipose tissue, mammary gland, testes, ovary, brain, kidney and muscle. Highest levels in liver.

Background: The GHR locus to human chromosome 5p13.1-p12 and to mouse chromosome 15. Additionally, its gene has 9 exons that encode the receptor and several additional exons in the 5-prime untranslated region. The coding exons span at least 87 kb. GHR consists of an extracellular domain of 246 amino acids, a single transmembrane domain, and a cytoplasmic domain. Exons 3 to 7 encode the extracellular domain. There are 2 isoforms of GHR in humans, generated by retention or exclusion of exon 3 during splicing: a full-length isoform and an isoform that lacks exon 3 (d3GHR). Furthermore, the two isoforms of GHR are expressed in the placenta and appeared to be due to alternative splicing. In cirrhosis, there is a state of acquired GH resistance, as defined by high circulating GH levels with low IGF1 levels. Moreover, Mutations in the GHR gene have been demonstrated as the cause of Laron syndrome , also known as the growth hormone insensitivity syndrome (GHIS).

Sequence Similarities: Belongs to the type I cytokine receptor family. Type 1 subfamily.

Molecular Weight: 72 kDa

UniProt: P16882

Pathways: NF-kappaB Signaling, JAK-STAT Signaling, Response to Growth Hormone Stimulus