SDHA antibody (N-Term)

Catalog No. ABIN3043074

The Rabbit Polyclonal anti-SDHA antibody (ABIN3043074) specifically detects SDHA in WB, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SDHA antibody (N-Term) (ABIN3043074)

Target: SDHA (Succinate Dehydrogenase Complex, Subunit A, Flavoprotein (Fp) (SDHA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SDHA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-SDHA Antibody

Binding Specificity: AA 113-128, N-Term

Purpose: Anti-SDHA Antibody

Sequence: NMEEDNWRWH FYDTVK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SDHA Antibody (ABIN3043074). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SDHA, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SDHA

Target: SDHA (Succinate Dehydrogenase Complex, Subunit A, Flavoprotein (Fp) (SDHA))

Alternative Name: SDHA

Background:

Synonyms: Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial,1.3.5.1,Flavoprotein subunit of complex II,Fp,SDHA,SDH2, SDHF,

Tissue Specificity: Wide tissue distribution. Undetectable in placental tissue.

Background: SDHA (Succinate dehydrogenase complex, subunit a, flavoprotein), also called SDH1, HOMOLOG OF, is a protein that in humans is encoded by the SDHA gene. The SDHA gene is mapped on 5p15.33. The SDHA gene is highly polymorphic. The gene that codes for the SDHA protein is nuclear, even though the protein is located in the inner membrane of the mitochondria. Heterozygous carriers of an SDHA mutation do not developparagangliomas as has been seen for mutations in the other subunits. In vitro functional expression studies in the yeast homolog showed that the mutation resulted in a loss of SDH activity and rendered the mutant SDHA protein more susceptible to proteolysis. The findings indicated that SDHA, like other SDH subunits, can act as a tumor suppressor gene.

Sequence Similarities: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Molecular Weight: 72 kDa

UniProt: P31040